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Sample GSM142427 Query DataSets for GSM142427
Status Public on Apr 27, 2007
Title abnormal eye & brain gene expression patterns in homozygous mbl/axin1 zebrafish - mbl 48hpf-B
Sample type RNA
 
Source name mbl/mbl 48 hr post-fertilization RNA replicate 2
Organism Danio rerio
Characteristics RNA obtained from mbl/mbl zebrafish embryos 48 hours post fertilization
Biomaterial provider Han Wang, PhD, University of Oklahoma
Treatment protocol Total RNA was obtained from approximately 50 embryos or larvae 24 hours post-fertilization
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy3
Label protocol Direct labeling incorporating Cy3-dUTP in cDNA from 2 micrograms total RNA with anchored oligo-dT priming and Omniscript reverse transcriptase
 
Hybridization protocol The labeled cDNA was added to ChipHybeTM hybridization buffer (Ventana Medical Systems, Tucson) containing Cot-1 DNA (0.5 mg/ml), yeast tRNA (0.2 mg/ml), and poly(dA)40-60 (0.4 mg/ml). Hybridization was performed on a Ventana Discovery system for 9 hr at 58C
Scan protocol Agilent DNA Microarray Scanner Model G2505B with fluorescent intensity was measured by Imageneā„¢ software
Description none
Data processing method of Dozmorov & Centola, Bioinformatics 19:204-211, 2003.
 
Submission date Oct 25, 2006
Last update date Apr 27, 2007
Contact name Bart Frank
E-mail(s) frank@omrf.org
Organization name OMRF
Street address 825 NE 13 St
City Oklahoma City
State/province OK
ZIP/Postal code 73103
Country USA
 
Platform ID GPL4481
Series (1)
GSE6142 Gene expression in homozygous mbl/axin1 zebrafish

Data table header descriptions
ID_REF
VALUE normalized/scaled expression level

Data table
ID_REF VALUE
001001001001 0.001158389
001001001002 0.001158389
001001001003 6.128343851
001001001004 0.001158389
001001001005 3.453057559
001001001006 4.322801724
001001001007 17.55256256
001001001008 0.001158389
001001001009 7.558160138
001001001010 13.96093649
001001001011 0.335662456
001001001012 0.001158389
001001001013 0.001158389
001001001014 34.82390092
001001001015 29.95137465
001001001016 5.930007334
001001001017 5.301749127
001001001018 6.350232406
001001001019 1.654508661
001001001020 0.001158389

Total number of rows: 14720

Table truncated, full table size 358 Kbytes.




Supplementary file Size Download File type/resource
GSM142427.txt.gz 164.5 Kb (ftp)(http) TXT

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