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Sample GSM1424855 Query DataSets for GSM1424855
Status Public on Jan 29, 2015
Title GerminalDiscRegion_hen69_MB
Sample type RNA
 
Source name GerminalDiscRegion_hen69_MB
Organism Gallus gallus
Characteristics tissue: germinal disc region
gender: Female
follicle stage: F1
Extracted molecule total RNA
Extraction protocol RNA from the blastodiscs of F1 follicles from the selected hens was purified using master pure RNA purification kit from the Epicentre (Cat. No. MCR85102) and following protocol as described by the manufacturer. All the 16 purified RNA samples were checked for integrity on the Agilent bioanalyzer according to Agilent Technologies Protocol. The amount of RNA isolated from the germinal disc region per hen ranged from 1.5-5 µg.
Label Cy3
Label protocol Labelling was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling. The input was 10 ng of total RNA and 600 ng of labelled cRNA was used on the 8 pack array.
 
Hybridization protocol Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). The hybridization temperature was 65°C with rotation speed 10 rpm for 17 hours. After 17 hours the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.
Scan protocol The arrays were scanned using the DNA microarray scanner with Surescan high resolution Technology from Agilent Technologies. Agilent Scan Control with resolution of 2µ, 16 bits and PMT of 100%.
Description Hen69_MaleBiased
Data processing Feature extraction was performed using protocol 10.7.3.1 (v10.7) for 1 colour gene expression.
 
Submission date Jul 02, 2014
Last update date Jan 29, 2015
Contact name Dirkjan Schokker
E-mail(s) dirkjan.schokker@wur.nl
Organization name Wageningen UR
Department Wageningen Livestock Research
Street address Droevendaalsesteeg 1
City Wageningen
ZIP/Postal code 6708 PB
Country Netherlands
 
Platform ID GPL18890
Series (1)
GSE59041 Association of egg mass and egg sex in chicken

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_87_P000014 4.616082596
A_87_P000029 11.10227537
A_87_P000043 3.866906992
A_87_P000046 5.132987631
A_87_P000062 7.349376985
A_87_P000100 4.083779836
A_87_P000117 5.951634183
A_87_P000138 4.083779836
A_87_P000145 4.82051887
A_87_P000163 7.952391755
A_87_P000174 4.883783224
A_87_P000199 4.083779836
A_87_P000253 6.918942603
A_87_P000268 3.471186942
A_87_P000312 4.688739308
A_87_P000340 5.047596114
A_87_P000349 2.607309128
A_87_P000372 3.315665117
A_87_P000379 8.60086685
A_87_P000386 8.12544786

Total number of rows: 43603

Table truncated, full table size 1060 Kbytes.




Supplementary file Size Download File type/resource
GSM1424855_US10513862_SLOT13_S01_GE1_107_Sep09_1_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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