|
| Status |
Public on Jan 29, 2015 |
| Title |
GerminalDiscRegion_hen99_MB |
| Sample type |
RNA |
| |
|
| Source name |
GerminalDiscRegion_hen99_MB
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: germinal disc region
gender: Female
follicle stage: F1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from the blastodiscs of F1 follicles from the selected hens was purified using master pure RNA purification kit from the Epicentre (Cat. No. MCR85102) and following protocol as described by the manufacturer. All the 16 purified RNA samples were checked for integrity on the Agilent bioanalyzer according to Agilent Technologies Protocol. The amount of RNA isolated from the germinal disc region per hen ranged from 1.5-5 µg.
|
| Label |
Cy3
|
| Label protocol |
Labelling was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling. The input was 10 ng of total RNA and 600 ng of labelled cRNA was used on the 8 pack array.
|
| |
|
| Hybridization protocol |
Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). The hybridization temperature was 65°C with rotation speed 10 rpm for 17 hours. After 17 hours the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.
|
| Scan protocol |
The arrays were scanned using the DNA microarray scanner with Surescan high resolution Technology from Agilent Technologies. Agilent Scan Control with resolution of 2µ, 16 bits and PMT of 100%.
|
| Description |
Hen99_FemaleBiased
|
| Data processing |
Feature extraction was performed using protocol 10.7.3.1 (v10.7) for 1 colour gene expression.
|
| |
|
| Submission date |
Jul 02, 2014 |
| Last update date |
Jan 29, 2015 |
| Contact name |
Dirkjan Schokker |
| E-mail(s) |
dirkjan.schokker@wur.nl
|
| Organization name |
Wageningen UR
|
| Department |
Wageningen Livestock Research
|
| Street address |
Droevendaalsesteeg 1
|
| City |
Wageningen |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL18890 |
| Series (1) |
| GSE59041 |
Association of egg mass and egg sex in chicken |
|
