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Sample GSM1424861 Query DataSets for GSM1424861
Status Public on Jan 29, 2015
Title GerminalDiscRegion_hen104_FB
Sample type RNA
 
Source name GerminalDiscRegion_hen104_FB
Organism Gallus gallus
Characteristics tissue: germinal disc region
gender: Female
follicle stage: F1
Extracted molecule total RNA
Extraction protocol RNA from the blastodiscs of F1 follicles from the selected hens was purified using master pure RNA purification kit from the Epicentre (Cat. No. MCR85102) and following protocol as described by the manufacturer. All the 16 purified RNA samples were checked for integrity on the Agilent bioanalyzer according to Agilent Technologies Protocol. The amount of RNA isolated from the germinal disc region per hen ranged from 1.5-5 µg.
Label Cy3
Label protocol Labelling was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling. The input was 10 ng of total RNA and 600 ng of labelled cRNA was used on the 8 pack array.
 
Hybridization protocol Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). The hybridization temperature was 65°C with rotation speed 10 rpm for 17 hours. After 17 hours the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent.
Scan protocol The arrays were scanned using the DNA microarray scanner with Surescan high resolution Technology from Agilent Technologies. Agilent Scan Control with resolution of 2µ, 16 bits and PMT of 100%.
Description Hen104_FemaleBiased
Data processing Feature extraction was performed using protocol 10.7.3.1 (v10.7) for 1 colour gene expression.
 
Submission date Jul 02, 2014
Last update date Jan 29, 2015
Contact name Dirkjan Schokker
E-mail(s) dirkjan.schokker@wur.nl
Organization name Wageningen UR
Department Wageningen Livestock Research
Street address Droevendaalsesteeg 1
City Wageningen
ZIP/Postal code 6708 PB
Country Netherlands
 
Platform ID GPL18890
Series (1)
GSE59041 Association of egg mass and egg sex in chicken

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_87_P000014 4.668080726
A_87_P000029 11.14448192
A_87_P000043 4.274757882
A_87_P000046 5.636817643
A_87_P000062 8.215403519
A_87_P000100 4.32840494
A_87_P000117 5.043956435
A_87_P000138 4.484781978
A_87_P000145 3.571675855
A_87_P000163 7.580874011
A_87_P000174 4.829003342
A_87_P000199 3.823408686
A_87_P000253 6.738632611
A_87_P000268 3.947041757
A_87_P000312 3.246416339
A_87_P000340 5.594647771
A_87_P000349 3.947041757
A_87_P000372 3.246416339
A_87_P000379 9.715144043
A_87_P000386 8.17927876

Total number of rows: 43603

Table truncated, full table size 1061 Kbytes.




Supplementary file Size Download File type/resource
GSM1424861_US10513862_SLOT13_S01_GE1_107_Sep09_2_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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