|
| Status |
Public on Oct 07, 2014 |
| Title |
CD3+_CD3_253 |
| Sample type |
genomic |
| |
|
| Source name |
CD3+ lymphocytes from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD3+ lymphocytes from peripheral blood
disease state: Myelodysplastic syndrome
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood prior to treatment with lenalidomide, at the individual centers participating in the study. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned according to the manufacturer's instructions.
|
| Description |
Hybridized to 6.0 Array
|
| Data processing |
CEL files, generated by AGCC Viewer. CNCHP and LOHCHP, generated by Genotyping Console (all provided by Affymetrix)
CNCHP (from 6.0 arrays) provide data for copy number and LOH analysis.
|
| |
|
| Submission date |
Jul 09, 2014 |
| Last update date |
Oct 07, 2014 |
| Contact name |
Vera Adema |
| E-mail(s) |
vera.adema@gmail.com
|
| Organization name |
Josep Carreras Leukaemia Research Institute
|
| Department |
MDS Group
|
| Street address |
Carretera de Can Ruti, Cami de les Escoles s/n
|
| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE59244 |
Affymetrix SNP array data for 5q- myelodysplastic syndromes |
|
