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Sample GSM1438951 Query DataSets for GSM1438951
Status Public on Feb 23, 2015
Title Tietar_leaves at T2 not irrigated
Sample type RNA
 
Source name Tietar T2 stress
Organism Zea mays
Characteristics variety: Tietar
developmental stage: silking (R1) (T2 stage)
growth condition: irrigation deficit
tissue: leaves
Treatment protocol Leaves were collected from plants at the vegetative eight-leaves (V8) (T1 stage) and at the silking (R1) (T2 stage), all at the same time of the day, immediately frozen in liquid nitrogen and stored at -80┬░C
Growth protocol Seeds were sown at a density of 80,000 plants/ha and were treated following standard agricultural practices. Each plot was fully irrigated until plants reached the vegetative six-leaf (V6) stage; from V6 to harvest they were irrigated differently : plot 1 was irrigated at 100% field capacity (control); plot 2 was not irrigated (0% field capacity, stress 2).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Spectrum® Plant Total RNA Kit (Sigma) according to the manufacturer's instruction
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Maize Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChip 3000 scanner device
Description STRESST
Data processing Partek Genomics Suite versione 6.5
 
Submission date Jul 17, 2014
Last update date Feb 24, 2015
Contact name Mariolina Gulli
Organization name University of Parma
Department Life Sciences
Street address Parco Area delle Scienze 11a
City Parma
ZIP/Postal code 43124
Country Italy
 
Platform ID GPL4032
Series (1)
GSE59533 Expression data from Zea mays cultivars Tietar and DKC 6575

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 246.6418
AFFX-BioB-M_at 353.4877
AFFX-BioB-3_at 311.0976
AFFX-BioC-5_at 832.9128
AFFX-BioC-3_at 1022.21
AFFX-BioDn-5_at 1865.997
AFFX-BioDn-3_at 4068.946
AFFX-CreX-5_at 12046.96
AFFX-CreX-3_at 12869.35
AFFX-DapX-5_at 161.5524
AFFX-DapX-M_at 753.3043
AFFX-DapX-3_at 1462.031
AFFX-LysX-5_at 101.5429
AFFX-LysX-M_at 122.168
AFFX-LysX-3_at 383.9658
AFFX-PheX-5_at 98.53346
AFFX-PheX-M_at 97.91488
AFFX-PheX-3_at 138.9277
AFFX-ThrX-5_at 29.25152
AFFX-ThrX-M_at 96.2729

Total number of rows: 17734

Table truncated, full table size 448 Kbytes.




Supplementary file Size Download File type/resource
GSM1438951_R736_6_STRESST_Maize_2.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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