|
| Status |
Public on Jul 22, 2014 |
| Title |
PC110 |
| Sample type |
SRA |
| |
|
| Source name |
glioma tissue - non-enhancing FLAIR+ sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: glioma - non-enhancing FLAIR+ sample
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated from the tissue specimens using the Qiagen TissueLyzer system with Qiazol and steel beads.
Poly(A)+ RNA-Seq libraries were constructed using the Illumina TruSeq v2 RNA-Seq kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
PC110
|
| Data processing |
Raw reads were aligned to hg19 using Tophat2 with --no-novel-juncs.
Expression levels (FPKM) were computed using Cufflinks2.
Genome_build: hg19
|
| |
|
| Submission date |
Jul 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter A Sims |
| E-mail(s) |
pas2182@columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
3960 Broadway, Lasker 203AC
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE59612 |
MRI-localized biopsies reveal subtype-specific differences in molecular and cellular composition at the margins of glioblastoma |
|
| Relations |
| BioSample |
SAMN02928513 |
| SRA |
SRX658003 |
