|
Status |
Public on Sep 09, 2014 |
Title |
hMSC AD D13 Rep2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
hMSC_adipocytic differentiation_day13
|
Organism |
Homo sapiens |
Characteristics |
cell type: human bone marrow MSCs (hMSC-TERT) time point: adipocytic differentiation for 13 days
|
Treatment protocol |
hMSC-TERT cells were cultured in basal medium in 24-well tissue culture plates. When cells reached 80-90% confluence, medium was replaced with Adipogenic induction medium (AIM) (DMEM medium supplemented with 10 % FBS, 10 % Horse Serum, 1 % Penicillin-Streptomycin, 100 nM dexamethasone, 0.45 mM isobutyl methyl xanthine (IBMX), 3 μg/mL insulin, and 1 μM Rosiglitazone. Control cells were cultured in parallel in normal DMEM medium. Cells pellets and RNA was extracted on days 0, 7, and 13.
|
Growth protocol |
Cells were grown in DMEM 4.5g/l glucose supplemented with 10% fetal bovine serum, 1% NEAA, 1% L-glutamine, 100 mg/l penicillin, and 100 mg/l streptomycin
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using TRIZOL method
|
Label |
Cy3
|
Label protocol |
The samples were labeled using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™
|
|
|
Channel 2 |
Source name |
common reference RNA pool
|
Organism |
Homo sapiens |
Characteristics |
sample type: equal amounts of all of the analyzed RNA samples
|
Treatment protocol |
hMSC-TERT cells were cultured in basal medium in 24-well tissue culture plates. When cells reached 80-90% confluence, medium was replaced with Adipogenic induction medium (AIM) (DMEM medium supplemented with 10 % FBS, 10 % Horse Serum, 1 % Penicillin-Streptomycin, 100 nM dexamethasone, 0.45 mM isobutyl methyl xanthine (IBMX), 3 μg/mL insulin, and 1 μM Rosiglitazone. Control cells were cultured in parallel in normal DMEM medium. Cells pellets and RNA was extracted on days 0, 7, and 13.
|
Growth protocol |
Cells were grown in DMEM 4.5g/l glucose supplemented with 10% fetal bovine serum, 1% NEAA, 1% L-glutamine, 100 mg/l penicillin, and 100 mg/l streptomycin
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using TRIZOL method
|
Label |
Hy5
|
Label protocol |
The samples were labeled using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™
|
|
|
|
Hybridization protocol |
Labeled RNA was hybridized on the miRCURY LNA™ microRNA Array (6'th GEN). Array product number 208400 Array version 6'th GEN, Array batch number 34014, miRBase version 17, GPL16968.
|
Scan protocol |
The miRCURY LNA™ microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon, Denmark).
|
Description |
microRNA expression on MSCs differentiated into adipocytes (day 13) AD D13 Rep2
|
Data processing |
The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
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|
|
Submission date |
Jul 23, 2014 |
Last update date |
Sep 09, 2014 |
Contact name |
Nehad M Alajez |
E-mail(s) |
nalajez@gmail.com
|
Organization name |
King Saud University College of Medicine
|
Department |
Anatomy
|
Lab |
Stem Cell Unit
|
Street address |
1st floor College of Medicne
|
City |
Riyadh |
ZIP/Postal code |
11461 |
Country |
Saudi Arabia |
|
|
Platform ID |
GPL17382 |
Series (1) |
GSE59684 |
microRNA expression profiling during Adipocytic differentiation of human MSCs (hMSC-TERT) |
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