|
| Status |
Public on Jan 01, 2007 |
| Title |
ABI-MRP2-3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult mouse retina pool 2
|
| Organism |
Mus musculus |
| Characteristics |
The adult mouse retina samples were obtained from pool 2 of C57/B6 mice (n = 350), which were purchased from Charles River Laboratories (Charles River Laboratories, Inc., Willmington, MA). All samples were placed in TRIzol reagent (Invitrogen, Carlsbad, CA) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. For a quality control measure of the samples, total RNA was ran through a 1% agarose gel and using the 2100 Agilent BioAnalyzer System (Agilent Technologies, Palo Alto, CA) to check for possible contamination and degradation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were dissected, collected and stored in Trizol (one pair of retinas per eppendorf tube) at -80°C prior to pooling.
|
| Label |
DIG-UTP
|
| Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
|
| |
|
| Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
|
| Description |
MRP2 technical replicate 3
|
| Data processing |
Signal intensities across microarrays were normalized using the quantile normalization (www.bioconductor.org).
|
| |
|
| Submission date |
Nov 18, 2006 |
| Last update date |
Dec 27, 2006 |
| Contact name |
Winston Patrick Kuo |
| E-mail(s) |
wkuo@genetics.med.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Cepko
|
| Street address |
188 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE6313 |
Comparison of Hybridization-based and Sequencing-based Gene Expression Technologies on Biological Replicates |
|
