|
| Status |
Public on Nov 06, 2007 |
| Title |
Papillary carcinoma, sample 98 |
| Sample type |
RNA |
| |
|
| Source name |
Homo sapiens thyroid, Papillary carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: female
Age: 23 years
Thyroïditis: no
Metastasis: yes
Nucleus type: Papillary
Nodule type: Multiple
Vesicle type: Papillary
Connective tissue type: Trabecular
Filter ID: 12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue samples using a standard guanidium isothiocyanate protocol (TRIzol reagent, Life Technologies, Inc., Gaithersburg, MD, USA)
|
| Label |
[alpha-33P]deoxy-CTP
|
| Label protocol |
the array was hybridized with a complex target prepared from 5 ?g total RNA by simultaneous RT and [alpha-33P]deoxy-CTP labelling as described by the manufacturer.
|
| |
|
| Hybridization protocol |
the array was hybridized with a complex target prepared from 5 ?g total RNA by simultaneous RT and [alpha-33P]deoxy-CTP labelling as described by the manufacturer.
|
| Scan protocol |
hybridization images were obtained by scanning with an imaging plate device (Fuji BAS 5000, Raytest, Paris, France). Signal intensities were quantified using ArrayGauge software (Fujifilm Medical Systems, Stanford, CT, USA).
|
| Description |
gene expression data from thyroid tissue diagnosed as Papillary carcinoma
|
| Data processing |
data were subjected to print-tip Lowess normalization and median normalization
|
| |
|
| Submission date |
Nov 21, 2006 |
| Last update date |
Nov 05, 2007 |
| Contact name |
Jean-Fred Fontaine |
| E-mail(s) |
jean-fred.fontaine@univ-angers.fr
|
| Phone |
+33 (0)2 41 35 33 14
|
| Fax |
+33 (0)2 41 35 40 17
|
| Organization name |
INSERM
|
| Lab |
Biochimie
|
| Street address |
4 rue Larrey
|
| City |
angers |
| ZIP/Postal code |
49033 |
| Country |
France |
| |
|
| Platform ID |
GPL4512 |
| Series (1) |
| GSE6339 |
Expression data from 12 human thyroid tissue classes and 2 cell lines |
|
