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Sample GSM1465208 Query DataSets for GSM1465208
Status Public on Mar 09, 2017
Title Normal_T319
Sample type RNA
 
Source name Normal prostate
Organism Homo sapiens
Characteristics tissue: normal prostate
gender: male
population: Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated following standard TriReagent protocol (Sigma-Aldrich) and DNase treatment was performed (TURBO DNase-free, Life Technologies). Total RNA quality and quantity were checked by means of Bioanalyzer (Agilent Technologies) and NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), respectively.
Label Cy3
Label protocol MiRNA expression profiling was carried out using the one-colour labelling method. Labelling, hybridization, washing and slide scanning were performed following the manufacturer's protocol (Agilent Technologies). Briefly, 100 ng of total RNA were dephosphorylated and denaturated; then a ligation and labeling step with Cy3 was performed.
 
Hybridization protocol Samples were hybridized to oligonucleotide glass arrays with sequences representing probes for 866 human and 89 human viral miRNAs from the Sanger miRBase (release 12.0) (Human miRNA Microarray V3, Agilent Technologies). After 20 hours, slides were washed and scanned.
Scan protocol Slides were scanned with the dual-laser Agilent scanner G2505B.
Description Peripheral region of the prostate from Caucasian men without PCa.
Data processing The LIMMA (LInear Models for Microarray Analysis) package was used to analyze miRNA expression profiles in normal prostates and prostate cancer tissues. Raw intensity values were background subtracted (method = subtract with an offset of 20) and normalized using the quantile method. Duplicated probes were averaged.
 
Submission date Aug 05, 2014
Last update date Mar 09, 2017
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL13264
Series (2)
GSE60117 miRNA expression profiling of prostate cancer (PCa) and normal prostatic samples
GSE60371 miR-424 induces COP1 silencing and STAT3 activation in prostate cancer: a novel miRNA-dependent axis driving tumor progression

Data table header descriptions
ID_REF
VALUE Log2 normalized intensity value

Data table
ID_REF VALUE
A_25_P00015264 12.68603571
A_25_P00011633 5.568498682
A_25_P00014956 5.494138848
A_25_P00011449 5.393690714
A_25_P00015155 5.459557725
A_25_P00013991 5.52167522
A_25_P00011357 5.637728335
A_25_P00012539 5.553791685
A_25_P00012520 5.576048686
A_25_P00011626 5.447269791
A_25_P00010708 5.511097928
A_25_P00011112 5.431355768
A_25_P00013618 5.565890323
A_25_P00010670 5.673473987
A_25_P00012491 5.466807072
A_25_P00015050 5.464164502
A_25_P00010807 6.420185291
A_25_P00012712 5.449738803
A_25_P00013670 5.54846507
A_25_P00011318 5.43220124

Total number of rows: 2689

Table truncated, full table size 70 Kbytes.




Supplementary file Size Download File type/resource
GSM1465208_US22502614_252182713984_S01_miRNA_107_Sep09_2_4.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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