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Sample GSM1465209 Query DataSets for GSM1465209
Status Public on Mar 09, 2017
Title Normal_T337
Sample type RNA
 
Source name Normal prostate
Organism Homo sapiens
Characteristics tissue: normal prostate
gender: male
population: Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated following standard TriReagent protocol (Sigma-Aldrich) and DNase treatment was performed (TURBO DNase-free, Life Technologies). Total RNA quality and quantity were checked by means of Bioanalyzer (Agilent Technologies) and NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), respectively.
Label Cy3
Label protocol MiRNA expression profiling was carried out using the one-colour labelling method. Labelling, hybridization, washing and slide scanning were performed following the manufacturer's protocol (Agilent Technologies). Briefly, 100 ng of total RNA were dephosphorylated and denaturated; then a ligation and labeling step with Cy3 was performed.
 
Hybridization protocol Samples were hybridized to oligonucleotide glass arrays with sequences representing probes for 866 human and 89 human viral miRNAs from the Sanger miRBase (release 12.0) (Human miRNA Microarray V3, Agilent Technologies). After 20 hours, slides were washed and scanned.
Scan protocol Slides were scanned with the dual-laser Agilent scanner G2505B.
Description Peripheral region of the prostate from Caucasian men without PCa.
Data processing The LIMMA (LInear Models for Microarray Analysis) package was used to analyze miRNA expression profiles in normal prostates and prostate cancer tissues. Raw intensity values were background subtracted (method = subtract with an offset of 20) and normalized using the quantile method. Duplicated probes were averaged.
 
Submission date Aug 05, 2014
Last update date Mar 09, 2017
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL13264
Series (2)
GSE60117 miRNA expression profiling of prostate cancer (PCa) and normal prostatic samples
GSE60371 miR-424 induces COP1 silencing and STAT3 activation in prostate cancer: a novel miRNA-dependent axis driving tumor progression

Data table header descriptions
ID_REF
VALUE Log2 normalized intensity value

Data table
ID_REF VALUE
A_25_P00015264 9.733698439
A_25_P00011633 5.452566451
A_25_P00014956 5.458947946
A_25_P00011449 5.41122492
A_25_P00015155 5.45243411
A_25_P00013991 5.591779358
A_25_P00011357 5.361864449
A_25_P00012539 5.528675223
A_25_P00012520 5.532904896
A_25_P00011626 5.454552431
A_25_P00010708 5.413933253
A_25_P00011112 5.499075953
A_25_P00013618 5.424372283
A_25_P00010670 5.501766204
A_25_P00012491 5.370501303
A_25_P00015050 5.386910429
A_25_P00010807 6.195698082
A_25_P00012712 5.449097237
A_25_P00013670 5.480522669
A_25_P00011318 5.370374894

Total number of rows: 2689

Table truncated, full table size 70 Kbytes.




Supplementary file Size Download File type/resource
GSM1465209_US22502614_252182714390_S01_miRNA_107_Sep09_2_1.txt.gz 2.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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