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Sample GSM1465211 Query DataSets for GSM1465211
Status Public on Mar 09, 2017
Title Normal_T218
Sample type RNA
Source name Normal prostate
Organism Homo sapiens
Characteristics tissue: normal prostate
gender: male
population: Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated following standard TriReagent protocol (Sigma-Aldrich) and DNase treatment was performed (TURBO DNase-free, Life Technologies). Total RNA quality and quantity were checked by means of Bioanalyzer (Agilent Technologies) and NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), respectively.
Label Cy3
Label protocol MiRNA expression profiling was carried out using the one-colour labelling method. Labelling, hybridization, washing and slide scanning were performed following the manufacturer's protocol (Agilent Technologies). Briefly, 100 ng of total RNA were dephosphorylated and denaturated; then a ligation and labeling step with Cy3 was performed.
Hybridization protocol Samples were hybridized to oligonucleotide glass arrays with sequences representing probes for 866 human and 89 human viral miRNAs from the Sanger miRBase (release 12.0) (Human miRNA Microarray V3, Agilent Technologies). After 20 hours, slides were washed and scanned.
Scan protocol Slides were scanned with the dual-laser Agilent scanner G2505B.
Description Peripheral region of the prostate from Caucasian men without PCa.
Data processing The LIMMA (LInear Models for Microarray Analysis) package was used to analyze miRNA expression profiles in normal prostates and prostate cancer tissues. Raw intensity values were background subtracted (method = subtract with an offset of 20) and normalized using the quantile method. Duplicated probes were averaged.
Submission date Aug 05, 2014
Last update date Mar 09, 2017
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
Platform ID GPL13264
Series (2)
GSE60117 miRNA expression profiling of prostate cancer (PCa) and normal prostatic samples
GSE60371 miR-424 induces COP1 silencing and STAT3 activation in prostate cancer: a novel miRNA-dependent axis driving tumor progression

Data table header descriptions
VALUE Log2 normalized intensity value

Data table
A_25_P00015264 11.22652471
A_25_P00011633 5.464279583
A_25_P00014956 5.478391474
A_25_P00011449 5.479485274
A_25_P00015155 5.398590794
A_25_P00013991 5.754568353
A_25_P00011357 5.406341378
A_25_P00012539 5.568369568
A_25_P00012520 5.692727333
A_25_P00011626 5.621179948
A_25_P00010708 5.473664712
A_25_P00011112 5.546676026
A_25_P00013618 5.410269936
A_25_P00010670 5.611278474
A_25_P00012491 5.405730414
A_25_P00015050 5.471721694
A_25_P00010807 6.075777431
A_25_P00012712 5.569458891
A_25_P00013670 5.588473609
A_25_P00011318 5.408833816

Total number of rows: 2689

Table truncated, full table size 70 Kbytes.

Supplementary file Size Download File type/resource
GSM1465211_US22502614_252182714390_S01_miRNA_107_Sep09_2_3.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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