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Sample GSM1465251 Query DataSets for GSM1465251
Status Public on Mar 09, 2017
Title PCa_G65
Sample type RNA
 
Source name Prostate cancer
Organism Homo sapiens
Characteristics tissue: prostate adenocarcinoma
gender: male
population: Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated following standard TriReagent protocol (Sigma-Aldrich) and DNase treatment was performed (TURBO DNase-free, Life Technologies). Total RNA quality and quantity were checked by means of Bioanalyzer (Agilent Technologies) and NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), respectively.
Label Cy3
Label protocol MiRNA expression profiling was carried out using the one-colour labelling method. Labelling, hybridization, washing and slide scanning were performed following the manufacturer's protocol (Agilent Technologies). Briefly, 100 ng of total RNA were dephosphorylated and denaturated; then a ligation and labeling step with Cy3 was performed.
 
Hybridization protocol Samples were hybridized to oligonucleotide glass arrays with sequences representing probes for 866 human and 89 human viral miRNAs from the Sanger miRBase (release 12.0) (Human miRNA Microarray V3, Agilent Technologies). After 20 hours, slides were washed and scanned.
Scan protocol Slides were scanned with the dual-laser Agilent scanner G2505B.
Description Prostate adenocarcinoma from Caucasian men.
Data processing The LIMMA (LInear Models for Microarray Analysis) package was used to analyze miRNA expression profiles in normal prostates and prostate cancer tissues. Raw intensity values were background subtracted (method = subtract with an offset of 20) and normalized using the quantile method. Duplicated probes were averaged.
 
Submission date Aug 05, 2014
Last update date Dec 18, 2019
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL13264
Series (2)
GSE60117 miRNA expression profiling of prostate cancer (PCa) and normal prostatic samples
GSE60371 miR-424 induces COP1 silencing and STAT3 activation in prostate cancer: a novel miRNA-dependent axis driving tumor progression
Relations
Reanalyzed by GSM4224482

Data table header descriptions
ID_REF
VALUE Log2 normalized intensity value

Data table
ID_REF VALUE
A_25_P00015264 13.77981648
A_25_P00011633 5.614908512
A_25_P00014956 5.573808791
A_25_P00011449 5.487304646
A_25_P00015155 5.555537661
A_25_P00013991 5.530907197
A_25_P00011357 5.650525437
A_25_P00012539 5.475915526
A_25_P00012520 5.633011516
A_25_P00011626 5.613552158
A_25_P00010708 5.451474292
A_25_P00011112 5.543676375
A_25_P00013618 5.430870965
A_25_P00010670 5.704534591
A_25_P00012491 5.447170717
A_25_P00015050 5.403808217
A_25_P00010807 5.798580657
A_25_P00012712 5.5513039
A_25_P00013670 5.559796549
A_25_P00011318 5.598884954

Total number of rows: 2689

Table truncated, full table size 70 Kbytes.




Supplementary file Size Download File type/resource
GSM1465251_miR-PCa_252182711939_S02_miRNA-v1_95_May07_1_3.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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