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Sample GSM1465262 Query DataSets for GSM1465262
Status Public on Mar 09, 2017
Title PCa_P55
Sample type RNA
Source name Prostate cancer
Organism Homo sapiens
Characteristics tissue: prostate adenocarcinoma
gender: male
population: Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated following standard TriReagent protocol (Sigma-Aldrich) and DNase treatment was performed (TURBO DNase-free, Life Technologies). Total RNA quality and quantity were checked by means of Bioanalyzer (Agilent Technologies) and NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), respectively.
Label Cy3
Label protocol MiRNA expression profiling was carried out using the one-colour labelling method. Labelling, hybridization, washing and slide scanning were performed following the manufacturer's protocol (Agilent Technologies). Briefly, 100 ng of total RNA were dephosphorylated and denaturated; then a ligation and labeling step with Cy3 was performed.
Hybridization protocol Samples were hybridized to oligonucleotide glass arrays with sequences representing probes for 866 human and 89 human viral miRNAs from the Sanger miRBase (release 12.0) (Human miRNA Microarray V3, Agilent Technologies). After 20 hours, slides were washed and scanned.
Scan protocol Slides were scanned with the dual-laser Agilent scanner G2505B.
Description Prostate adenocarcinoma from Caucasian men.
Data processing The LIMMA (LInear Models for Microarray Analysis) package was used to analyze miRNA expression profiles in normal prostates and prostate cancer tissues. Raw intensity values were background subtracted (method = subtract with an offset of 20) and normalized using the quantile method. Duplicated probes were averaged.
Submission date Aug 05, 2014
Last update date Dec 18, 2019
Contact name Paola Ostano
Organization name Fondazione Edo ed Elvo Tempia
Street address via Malta 3
City Biella
ZIP/Postal code 13900
Country Italy
Platform ID GPL13264
Series (2)
GSE60117 miRNA expression profiling of prostate cancer (PCa) and normal prostatic samples
GSE60371 miR-424 induces COP1 silencing and STAT3 activation in prostate cancer: a novel miRNA-dependent axis driving tumor progression
Reanalyzed by GSM4224489

Data table header descriptions
VALUE Log2 normalized intensity value

Data table
A_25_P00015264 12.64751526
A_25_P00011633 5.447396263
A_25_P00014956 5.480229703
A_25_P00011449 5.512632093
A_25_P00015155 5.518978447
A_25_P00013991 5.571829076
A_25_P00011357 5.595108523
A_25_P00012539 5.462326504
A_25_P00012520 5.507598973
A_25_P00011626 5.550121677
A_25_P00010708 5.515461071
A_25_P00011112 5.40843526
A_25_P00013618 5.397546536
A_25_P00010670 5.782510294
A_25_P00012491 5.50476536
A_25_P00015050 5.46188779
A_25_P00010807 5.825318742
A_25_P00012712 5.521572135
A_25_P00013670 5.529983648
A_25_P00011318 5.601927708

Total number of rows: 2689

Table truncated, full table size 70 Kbytes.

Supplementary file Size Download File type/resource
GSM1465262_miR-PCa_252182711940_S01_miRNA-v1_95_May07_2_3.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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