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Sample GSM146789 Query DataSets for GSM146789
Status Public on Nov 23, 2006
Title Stage 2, PT#8, Normal (HG-U133A)
Sample type RNA
Source name Human Renal Epithelium
Organism Homo sapiens
Characteristics Normal, PT#8, STG2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
Hybridization protocol Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C on HG U133A and HG U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description Normal HRE
Data processing The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500
Submission date Nov 22, 2006
Last update date Nov 22, 2006
Contact name John A Copland
Organization name Mayo Clinic College of Medicine
Department Biochemistry & Molecular Biology
Street address 4500 San Pablo Road
City Jacksonville
State/province FL
ZIP/Postal code 32224
Country USA
Platform ID GPL96
Series (1)
GSE6344 Gene expression in Stage 1,2 Normal and Tumor kidney cancer

Data table header descriptions
VALUE MAS5-calculated Signal intensity

Data table
AFFX-BioB-5_at 560.6 P 0.006187
AFFX-BioB-M_at 978 P 0.000195
AFFX-BioB-3_at 640.5 P 0.000857
AFFX-BioC-5_at 1343.7 P 0.000127
AFFX-BioC-3_at 1311.1 P 0.00011
AFFX-BioDn-5_at 1185.5 P 6.00E-05
AFFX-BioDn-3_at 8626.8 P 5.20E-05
AFFX-CreX-5_at 14430.1 P 4.40E-05
AFFX-CreX-3_at 19156.1 P 4.40E-05
AFFX-DapX-5_at 53.9 A 0.131361
AFFX-DapX-M_at 69.9 M 0.062929
AFFX-DapX-3_at 68 A 0.368438
AFFX-LysX-5_at 1788.5 P 4.40E-05
AFFX-LysX-M_at 4432.1 P 4.40E-05
AFFX-LysX-3_at 8170 P 4.40E-05
AFFX-PheX-5_at 4079.3 P 4.40E-05
AFFX-PheX-M_at 9238.2 P 4.40E-05
AFFX-PheX-3_at 12100.1 P 4.40E-05
AFFX-ThrX-5_at 102.8 M 0.050229
AFFX-ThrX-M_at 285.5 P 0.012547

Total number of rows: 22283

Table truncated, full table size 594 Kbytes.

Supplementary file Size Download File type/resource
GSM146789.CEL.gz 3.4 Mb (ftp)(http) CEL

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