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Sample GSM146795 Query DataSets for GSM146795
Status Public on Nov 23, 2006
Title Stage 2, PT#11, Tumor (HG-U133A)
Sample type RNA
Source name Human Renal Epithelium
Organism Homo sapiens
Characteristics Tumor, PT#11, STG2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
Hybridization protocol Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C on HG U133A and HG U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description Tumor HRE
Data processing The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500
Submission date Nov 22, 2006
Last update date Nov 22, 2006
Contact name John A Copland
Organization name Mayo Clinic College of Medicine
Department Biochemistry & Molecular Biology
Street address 4500 San Pablo Road
City Jacksonville
State/province FL
ZIP/Postal code 32224
Country USA
Platform ID GPL96
Series (1)
GSE6344 Gene expression in Stage 1,2 Normal and Tumor kidney cancer

Data table header descriptions
VALUE MAS5-calculated Signal intensity

Data table
AFFX-BioB-5_at 316.1 P 0.00762
AFFX-BioB-M_at 513.4 P 0.000195
AFFX-BioB-3_at 328.8 P 0.002867
AFFX-BioC-5_at 1152.1 P 0.000169
AFFX-BioC-3_at 716.7 P 0.00011
AFFX-BioDn-5_at 922.7 P 8.10E-05
AFFX-BioDn-3_at 5229.1 P 9.50E-05
AFFX-CreX-5_at 8715.1 P 4.40E-05
AFFX-CreX-3_at 12762.1 P 4.40E-05
AFFX-DapX-5_at 59.9 P 0.010317
AFFX-DapX-M_at 103 P 0.028457
AFFX-DapX-3_at 60.6 A 0.313723
AFFX-LysX-5_at 2860.8 P 4.40E-05
AFFX-LysX-M_at 5290.7 P 4.40E-05
AFFX-LysX-3_at 7026.1 P 4.40E-05
AFFX-PheX-5_at 10336.5 P 4.40E-05
AFFX-PheX-M_at 12091 P 4.40E-05
AFFX-PheX-3_at 14289.8 P 4.40E-05
AFFX-ThrX-5_at 263.9 P 0.002867
AFFX-ThrX-M_at 378.9 P 0.001102

Total number of rows: 22283

Table truncated, full table size 595 Kbytes.

Supplementary file Size Download File type/resource
GSM146795.CEL.gz 3.4 Mb (ftp)(http) CEL

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