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Sample GSM146800 Query DataSets for GSM146800
Status Public on Nov 23, 2006
Title Stage 1, PT#3, Normal (HG-U133B)
Sample type RNA
Source name Human Renal Epithelium
Organism Homo sapiens
Characteristics Normal, PT#3, STG1
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
Hybridization protocol Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C on HG U133A and HG U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description Normal HRE
Data processing The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500
Submission date Nov 22, 2006
Last update date Nov 22, 2006
Contact name John A Copland
Organization name Mayo Clinic College of Medicine
Department Biochemistry & Molecular Biology
Street address 4500 San Pablo Road
City Jacksonville
State/province FL
ZIP/Postal code 32224
Country USA
Platform ID GPL97
Series (1)
GSE6344 Gene expression in Stage 1,2 Normal and Tumor kidney cancer

Data table header descriptions
VALUE MAS5-calculated Signal intensity

Data table
AFFX-BioB-5_at 1853 P 0.002867
AFFX-BioB-M_at 3174.2 P 0.000509
AFFX-BioB-3_at 1464.6 P 0.000581
AFFX-BioC-5_at 4378.7 P 0.00011
AFFX-BioC-3_at 4229.1 P 0.000147
AFFX-BioDn-5_at 3825 P 5.20E-05
AFFX-BioDn-3_at 23359 P 5.20E-05
AFFX-CreX-5_at 41484.5 P 4.40E-05
AFFX-CreX-3_at 67756 P 4.40E-05
AFFX-DapX-5_at 126.3 A 0.300606
AFFX-DapX-M_at 308.8 A 0.195266
AFFX-DapX-3_at 64 A 0.60308
AFFX-LysX-5_at 2509 P 4.40E-05
AFFX-LysX-M_at 10820.3 P 4.40E-05
AFFX-LysX-3_at 17379.9 P 6.00E-05
AFFX-PheX-5_at 7845.1 P 4.40E-05
AFFX-PheX-M_at 19108.6 P 4.40E-05
AFFX-PheX-3_at 31346.9 P 4.40E-05
AFFX-ThrX-5_at 75.3 A 0.300606
AFFX-ThrX-M_at 610 P 0.042962

Total number of rows: 22645

Table truncated, full table size 593 Kbytes.

Supplementary file Size Download File type/resource
GSM146800.CEL.gz 3.2 Mb (ftp)(http) CEL

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