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Sample GSM146816 Query DataSets for GSM146816
Status Public on Nov 23, 2006
Title Stage 2, PT#12, Normal (HG-U133B)
Sample type RNA
Source name Human Renal Epithelium
Organism Homo sapiens
Characteristics Normal, PT#12, STG2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
Hybridization protocol Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C on HG U133A and HG U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description Normal HRE
Data processing The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500
Submission date Nov 22, 2006
Last update date Nov 22, 2006
Contact name John A Copland
Organization name Mayo Clinic College of Medicine
Department Biochemistry & Molecular Biology
Street address 4500 San Pablo Road
City Jacksonville
State/province FL
ZIP/Postal code 32224
Country USA
Platform ID GPL97
Series (1)
GSE6344 Gene expression in Stage 1,2 Normal and Tumor kidney cancer

Data table header descriptions
VALUE MAS5-calculated Signal intensity

Data table
AFFX-BioB-5_at 1486.9 P 0.002023
AFFX-BioB-M_at 3108.8 P 0.000195
AFFX-BioB-3_at 1181.9 P 0.000857
AFFX-BioC-5_at 4294.7 P 6.00E-05
AFFX-BioC-3_at 3305.9 P 7.00E-05
AFFX-BioDn-5_at 3333 P 8.10E-05
AFFX-BioDn-3_at 20733 P 5.20E-05
AFFX-CreX-5_at 39955.1 P 4.40E-05
AFFX-CreX-3_at 56718 P 4.40E-05
AFFX-DapX-5_at 283.3 P 0.028457
AFFX-DapX-M_at 232.9 A 0.250796
AFFX-DapX-3_at 156.5 A 0.814869
AFFX-LysX-5_at 6767.6 P 4.40E-05
AFFX-LysX-M_at 14284.5 P 4.40E-05
AFFX-LysX-3_at 22122.5 P 4.40E-05
AFFX-PheX-5_at 21300 P 4.40E-05
AFFX-PheX-M_at 30367.2 P 4.40E-05
AFFX-PheX-3_at 30691 P 4.40E-05
AFFX-ThrX-5_at 531.9 P 0.001248
AFFX-ThrX-M_at 1081.4 P 0.000754

Total number of rows: 22645

Table truncated, full table size 593 Kbytes.

Supplementary file Size Download File type/resource
GSM146816.CEL.gz 3.2 Mb (ftp)(http) CEL

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