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Status |
Public on Oct 24, 2008 |
Title |
Xylem Pole Pericycle Cells at T0, biological rep2 |
Sample type |
RNA |
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Source name |
Arabidopsis roots at 72 HAG on NPA
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Organism |
Arabidopsis thaliana |
Characteristics |
Genotype: J0121 (for more information, please see the Dept. of Plant Sciences website of GAL4-GFP expressino patterns at the University of Cambridge) Age seedlings: 72 HAG
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Treatment protocol |
Roots were treated according to Birnbaum et al (2002, 2005) to isolate xylem pole pericycle cells, which were frozen in RLT buffer
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Growth protocol |
Seedlings were grown on MS supplemented with 10 microM NPA (as described by Himanen et al., 2002) on top of nylon mesh. The seedlings were grown in 16h/8h day/night conditions at 22 C
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from frozen material using the Qiagen RNeasy kit (Valencia, California, United States) according to the manufacturer's instructions
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from undetectable amouns of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, cRNA was hybridized on GeneChip Arabidopsis ATH1 Genome Array. Treatments were done according to the protocol on the Duke Microarray Facility web site
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Scan protocol |
GeneChips were scanned using the Axon GenePix 4000B scanner (Molecular Devices)
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Description |
Gene expression data from xylem pole pericycle cells from 72-HAG-old Arabidopsis roots grown on NPA
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Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
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Submission date |
Nov 22, 2006 |
Last update date |
Oct 14, 2008 |
Contact name |
Ive De Smet |
E-mail(s) |
ive.desmet@zmbp.uni-tuebingen.de
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Organization name |
University of Tübingen - Center for Plant Molecular Biology
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Street address |
Auf der Morgenstelle 3
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City |
Tuebingen |
ZIP/Postal code |
D-72076 |
Country |
Germany |
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Platform ID |
GPL198 |
Series (1) |
GSE6349 |
Expression data from xylem pole pericycle cells of Arabidopsis roots undergoing lateral root initiation |
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