|
| Status |
Public on Oct 24, 2008 |
| Title |
Xylem Pole Pericycle Cells at T2, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis roots at 72 HAG on NPA + 2h NAA
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Genotype: J0121 (for more information, please see the Dept. of Plant Sciences website of GAL4-GFP expressino patterns at the University of Cambridge)
Age seedlings: 74 HAG
|
| Treatment protocol |
Roots were treated according to Birnbaum et al (2002, 2005) to isolate xylem pole pericycle cells, which were frozen in RLT buffer
|
| Growth protocol |
Seedlings were grown on MS supplemented with 10 microM NPA (as described by Himanen et al., 2002) on top of nylon mesh. The seedlings were grown in 16h/8h day/night conditions at 22 C. At 72 HAG the seedlings were transfered to 10 microM NAA for 2h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen material using the Qiagen RNeasy kit (Valencia, California, United States) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from undetectable amouns of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on GeneChip Arabidopsis ATH1 Genome Array. Treatments were done according to the protocol on the Duke Microarray Facility web site
|
| Scan protocol |
GeneChips were scanned using the Axon GenePix 4000B scanner (Molecular Devices)
|
| Description |
Gene expression data from xylem pole pericycle cells from 72-HAG-old Arabidopsis roots grown on NPA and 2h NAA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0)
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| |
|
| Submission date |
Nov 22, 2006 |
| Last update date |
Oct 14, 2008 |
| Contact name |
Ive De Smet |
| E-mail(s) |
ive.desmet@zmbp.uni-tuebingen.de
|
| Organization name |
University of Tübingen - Center for Plant Molecular Biology
|
| Street address |
Auf der Morgenstelle 3
|
| City |
Tuebingen |
| ZIP/Postal code |
D-72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE6349 |
Expression data from xylem pole pericycle cells of Arabidopsis roots undergoing lateral root initiation |
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