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Sample GSM148061 Query DataSets for GSM148061
Status Public on Apr 01, 2007
Title gDNA_EColi_H27
Sample type genomic
 
Channel 1
Source name gDNA from E.coli K12 cyanine 3
Organism Escherichia coli K-12
Characteristics gDNA K12
Extracted molecule genomic DNA
Extraction protocol DNeasy (Qiagen)
Label cyanine 3
Label protocol Klenow and random primers
 
Channel 2
Source name strain H27
Organism Escherichia coli
Characteristics gDNA H27
Extracted molecule genomic DNA
Extraction protocol DNeasy (Qiagen)
Label Cyanine 5
Label protocol Klenow and random primers
 
 
Hybridization protocol Agilent standard protocol 17H at 60 degrees C; Agilent proprietary Buffers
Scan protocol Images were quantified using Agilent Feature Extraction Software.
Description Comparison of gDNA K12/H27
Data processing Local Background Substracted Signal Corrected for unequal Dye incorporation or unequal load of labelled product
 
Submission date Dec 04, 2006
Last update date Dec 18, 2006
Contact name FRANCOIS Patrice
E-mail(s) patrice.francois@genomic.ch
Phone +41 (0)22 372 93 37
Organization name Genomic Research Laboratory
Department Service of Infectious Diseases
Lab Genomic Research Lab
Street address Rue Gabrielle-Perret-Gentil, 4
City Geneva
State/province Ge 4
ZIP/Postal code 1211
Country Switzerland
 
Platform ID GPL4618
Series (1)
GSE6486 Comparative genomic hybridization and physiological characterization of environmental E.coli

Data table header descriptions
ID_REF Agilent Feature ID number
VALUE log10-transformed ratios of rBGSubSignal (Cy-5, channel 2) divided by gBGSubSignal (Cy-3, channel 1)
gBGSubSignal Cy-3 Background Substracted Signal (Median Normalized)
rBGSubSignal Cy-5 Background Substracted Signal (Median Normalized)

Data table
ID_REF VALUE gBGSubSignal rBGSubSignal
1 1 1
2 1 1
3 1 1
4 1 1
5 -0.269370153 17902.1 9627.95
6 -0.554991905 10243.8 2854.1
7 1 1
8 1 1
9 1 1
10 1 1
11 1 1
12 1 1
13 1 1
14 1 1
15 1 1
16 1 1
17 1 1
18 1 1
19 1 1
20 1 1

Total number of rows: 2753

Table truncated, full table size 87 Kbytes.




Supplementary file Size Download File type/resource
GSM148061.txt.gz 75.8 Kb (ftp)(http) TXT

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