|
| Status |
Public on Dec 30, 2014 |
| Title |
CD34+/CD1a- technical replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
thymus biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: mixed
age: 1-24 months
cell type: CD34+/CD1a-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Normal human postnatal thymocytes were isolated by Ficoll-Hypaque centrifugation and magnetic cell sorting from thymus fragments removed during corrective cardiac surgery
|
| Label |
Cy3
|
| Label protocol |
TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System, Epicentre (Cat.Num. TAN07924)
|
| |
|
| Hybridization protocol |
Whole-Genome Gene Expression Direct Hybridization Illumina Inc.'s protocol
|
| Scan protocol |
BeadScan Illumina Inc.
|
| Description |
34_37_1
|
| Data processing |
Raw data were decoded by GenomeStudio Illumina Inc. And normalization was done (1) forcePositive background correction, (2) log2 transformation and (3) quantile normalization
|
| |
|
| Submission date |
Aug 21, 2014 |
| Last update date |
Dec 30, 2014 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
|
| Phone |
0034944061325
|
| Organization name |
CIC bioGUNE
|
| Department |
Genome Analysis Platform
|
| Street address |
Parque tecnologico de Bizkaia, Building 801-A
|
| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE54996 |
DNA METHYLATION DURING αβ T CELL DEVELOPMENT IN THE HUMAN THYMUS [expression] |
| GSE55112 |
DNA METHYLATION DURING αβ T CELL DEVELOPMENT IN THE HUMAN THYMUS |
|
