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Sample GSM149656 Query DataSets for GSM149656
Status Public on Nov 08, 2007
Title Dsim_male_rep2
Sample type RNA
 
Source name Drosophila simulans adult male
Organism Drosophila simulans
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 2 of 4.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4632
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2212_0001_0001 9.621 1044.44
2212_0004_0001 15.381 45154.11
2212_0006_0001 15.285 42273.78
2212_0008_0001 15.161 38801.44
2212_0010_0001 15.552 50823.89
2212_0012_0001 15.668 55062.22
2212_0014_0001 15.641 54045.11
2212_0016_0001 15.597 52418.22
2212_0018_0001 15.453 47463.44
2212_0020_0001 15.749 58218.11
2212_0022_0001 15.682 55572.11
2212_0025_0001 11.075 2487.33
2212_0027_0001 9.877 1205.22
2212_0029_0001 9.767 1132.67
2212_0031_0001 10.386 1624.78
2212_0033_0001 9.427 939.67
2212_0035_0001 10.767 2051.33
2212_0037_0001 9.655 1064.22
2212_0039_0001 10.562 1807.78
2212_0041_0001 12.229 5272.44

Total number of rows: 390209

Table truncated, full table size 10688 Kbytes.




Supplementary file Size Download File type/resource
GSM149656.ftr.gz 8.4 Mb (ftp)(http) FTR
Processed data included within Sample table

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