NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM149712 Query DataSets for GSM149712
Status Public on Nov 08, 2007
Title Dvir_female_rep1
Sample type RNA
 
Source name Drosophila virilis adult female
Organism Drosophila virilis
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 1 of 4.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4633
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2213_0001_0001 9.791 931.33
2213_0004_0001 16 65535
2213_0006_0001 15.998 65439
2213_0008_0001 15.946 63125.44
2213_0010_0001 16 65535
2213_0012_0001 16 65535
2213_0014_0001 16 65535
2213_0016_0001 16 65535
2213_0018_0001 16 65535
2213_0020_0001 16 65535
2213_0022_0001 16 65535
2213_0025_0001 8.204 340.33
2213_0027_0001 9.753 907.78
2213_0029_0001 14.064 17161.56
2213_0031_0001 7.388 213.11
2213_0033_0001 9.423 731.56
2213_0035_0001 9.54 789.67
2213_0037_0001 12.66 6514.44
2213_0039_0001 10.456 1449.67
2213_0041_0001 11.661 3281

Total number of rows: 391015

Table truncated, full table size 10928 Kbytes.




Supplementary file Size Download File type/resource
GSM149712.ftr.gz 8.4 Mb (ftp)(http) FTR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap