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Sample GSM149713 Query DataSets for GSM149713
Status Public on Nov 08, 2007
Title Dvir_female_rep2
Sample type RNA
 
Source name Drosophila virilis adult female
Organism Drosophila virilis
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 2 of 4.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4633
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2213_0001_0001 9.795 965.33
2213_0004_0001 15.862 61923.22
2213_0006_0001 15.891 63208.67
2213_0008_0001 15.811 59785.33
2213_0010_0001 15.646 53349.78
2213_0012_0001 15.747 57181.33
2213_0014_0001 15.857 61726.33
2213_0016_0001 15.88 62729.89
2213_0018_0001 15.797 59200.56
2213_0020_0001 15.609 51973.89
2213_0022_0001 15.805 59561.33
2213_0025_0001 7.234 198.22
2213_0027_0001 8.848 520.78
2213_0029_0001 15.055 35418.78
2213_0031_0001 6.471 133.89
2213_0033_0001 8.863 525.78
2213_0035_0001 8.701 474.33
2213_0037_0001 12.505 6082.89
2213_0039_0001 9.695 903.22
2213_0041_0001 10.986 2149.67

Total number of rows: 391015

Table truncated, full table size 10962 Kbytes.




Supplementary file Size Download File type/resource
GSM149713.ftr.gz 8.4 Mb (ftp)(http) FTR
Processed data included within Sample table

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