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Sample GSM149714 Query DataSets for GSM149714
Status Public on Nov 08, 2007
Title Dvir_female_rep3
Sample type RNA
 
Source name Drosophila virilis adult female
Organism Drosophila virilis
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 3 of 4.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4633
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2213_0001_0001 9.865 247.44
2213_0004_0001 17.126 29635.11
2213_0006_0001 17.215 31524.89
2213_0008_0001 17.345 34496.78
2213_0010_0001 17.286 33104.44
2213_0012_0001 17.193 31043.56
2213_0014_0001 17.378 35287.22
2213_0016_0001 17.439 36807.78
2213_0018_0001 17.442 36884.22
2213_0020_0001 17.443 36916.67
2213_0022_0001 17.282 33016.78
2213_0025_0001 8.221 116.33
2213_0027_0001 8.682 139.56
2213_0029_0001 14.004 3452.67
2213_0031_0001 7.458 91
2213_0033_0001 9.3 185
2213_0035_0001 9.054 164.56
2213_0037_0001 11.644 716.56
2213_0039_0001 10.125 285.56
2213_0041_0001 11.868 827.78

Total number of rows: 391015

Table truncated, full table size 10757 Kbytes.




Supplementary file Size Download File type/resource
GSM149714.ftr.gz 8.1 Mb (ftp)(http) FTR
Processed data included within Sample table

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