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Sample GSM149828 Query DataSets for GSM149828
Status Public on Mar 01, 2007
Title hy5 mutant vs wildtype expression, rep1
Sample type RNA
 
Channel 1
Source name wildtype reference
Organism Arabidopsis thaliana
Characteristics 4 days wildtype seedling
Growth protocol continuous white light for 4 days
Extracted molecule total RNA
Extraction protocol Trizol/RNeasy
Label Cy5
Label protocol amino-allyl and monofunctional dye
 
Channel 2
Source name hy5 mutant
Organism Arabidopsis thaliana
Characteristics 4 days hy5 mutant seedling
Extracted molecule total RNA
Extraction protocol Trizol/RNeasy
Label Cy3
Label protocol amino-allyl and monofunctional dye
 
 
Hybridization protocol 42C 50% formamide
Scan protocol Axon GenePix 4000B
Description hy5 mutant vs. wildtype
Data processing LIMMA in bioconductor R package
 
Submission date Dec 13, 2006
Last update date Dec 13, 2006
Contact name Kun He
E-mail(s) hek@mail.cbi.pku.edu.cn
Phone 203-432-8909
Organization name Yale University
Department Molecular, Cellular & Developmental Biology
Lab Denglab
Street address 165 Prospect
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL988
Series (1)
GSE6517 expression profiling of wild type and the hy5 mutant in arabidopsis

Data table header descriptions
ID_REF
VALUE normalized log ratio of CH1/CH2
CH1_SIG_MEAN Channel 1 (Cy5, wildtype) mean pixel intensity
CH1_BKG_MEDIAN Channel 1 Background median pixel intensity
CH2_SIG_MEAN Channel 2 (Cy3, hy5 mutant) mean pixel intensity
CH2_BKG_MEDIAN Channel 2 Background median pixel intensity

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKG_MEDIAN CH2_SIG_MEAN CH2_BKG_MEDIAN
3203159 -1.45936212003885 351 332 341 325
3203161 0.0952733719830288 733 335 826 327
3203163 1.08530582536591 387 327 334 319
3203165 0.875753701527684 406 329 364 328
3203167 -2.55884882425440 351 340 337 324
3203169 0.115966127107934 403 340 398 347
3203171 null 348 348 377 371
3203173 -0.683087227444034 414 333 518 366
3203175 -1.56536570108287 359 343 363 354
3203177 -0.093964860239373 588 343 676 335
3203179 0.0204217625456807 809 331 963 312
3203181 0.199605462657085 528 328 535 317
3203183 -0.866930354315111 373 330 397 324
3203185 -1.25064763498350 359 334 359 319
3203187 -1.04373540413407 354 327 373 338
3203189 -2.70812913160879 340 327 372 334
3203191 1.39733409788672 391 325 343 332
3203193 -0.650587463730365 373 335 383 326
3203195 -0.151666718322095 467 340 504 327
3203197 0.051766599658321 434 331 445 337

Total number of rows: 27648

Table truncated, full table size 1120 Kbytes.




Supplementary data files not provided
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