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Sample GSM1500 Query DataSets for GSM1500
Status Public on Apr 18, 2002
Title prp4-TempShift-0mins_5
Sample type RNA
 
Channel 1
Source name SS330 (wt) @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name SRY4-1a (prp4-1) @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Prp4-1 and wt strains were grown at 26deg to A600 of 1.0, then an equal volume of 48deg media was added to bring the temperature to 37deg. Both strains were allowed to grow at 37deg and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature.
Lot batch = Slide4-32
 
Submission date Mar 19, 2002
Last update date May 27, 2005
Contact name Manuel Ares
E-mail(s) ares@biology.ucsc.edu
Phone 831 459-4628
Organization name UC Santa Cruz
Street address
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platform ID GPL108
Series (1)
GSE34 Prp4-1 temperature shift

Data table header descriptions
ID_REF
X_COORD X-coordinate of spot
Y_COORD Y-coordinate of spot
SPOT_SIZE Size of the spot (diameter, in microns)
CH2_MEDIAN Channel 2 (Cy5/635) median pixel intensity - feature
CH2_MEAN Channel 2 (Cy5/635) mean pixel intensity - feature
CH2_SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
CH2_BKD_MEDIAN Channel 2 (Cy5/635) median background intensity
CH2_BKD_MEAN Channel 2 (Cy5/635) mean background intensity
CH2_BKD_SD Channel 2 (Cy5/635) standard deviation of background
CH2_PERCNT_PIX_1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
CH2_PERCNT_PIX_2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
CH2_PERCNT_PIX_SAT Channel 2 (Cy5/635) percent of pixels saturated
CH1_MEDIAN Channel 1 (Cy3/532) median pixel intensity - feature
CH1_MEAN Channel 1 (Cy3/532) mean pixel intensity - feature
CH1_SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
CH1_BKD_MEDIAN Channel 1 (Cy3/532) median background intensity
CH1_BKD_MEAN Channel 1 (Cy3/532) mean background intensity
CH1_BKD_SD Channel 1 (Cy3/532) standard deviation of background
CH1_PERCNT_PIX_1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
CH1_PERCNT_PIX_2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
CH1_PERCNT_PIX_SAT Channel 1 (Cy3/532) percent of pixels saturated
RATIO_MEDIANS Ratio of medians (Ch2/Ch1)
RATIO_MEANS Ratio of means (Ch2/Ch1)
MEDIAN_RATIOS Median of ratios
MEAN_RATIOS Mean of ratios
RATIO_SD Ratio standard deviation
RGN_RATIO Regression ratio
RGN_SQ_RATIO Regression square ratio
SPOT_PIXELS Number of feature pixels
BKD_PIXELS Number of background pixels
LOG_RATIO Log ratio (base 2)
CH2_MED_MINUS_BKD Channel 2 (Cy5/635) background subtracted median feature intensity
CH1_MED_MINUS_BKD Channel 1 (Cy3/532) background subtracted median feature intensity
CH2_MEAN_MINUS_BKD Channel 2 (Cy5/635) background subtracted mean feature intensity
CH1_MEAN_MINUS_BKD Channel 1 (Cy3/532) background subtracted mean feature intensity
FLAG User defined flag (default=0)
NORM_RATIO Normalized ratio of medians
VALUE Normalized log ratio (base 2)
MEAN_VALUE Average normalized log ratio for replicate spots
SD_VALUE Standard deviation of average normalized log ratio
NUM_REPLICATES Number of replicate spots used in average normalized log ratio

Data table
ID_REF X_COORD Y_COORD SPOT_SIZE CH2_MEDIAN CH2_MEAN CH2_SD CH2_BKD_MEDIAN CH2_BKD_MEAN CH2_BKD_SD CH2_PERCNT_PIX_1SD CH2_PERCNT_PIX_2SD CH2_PERCNT_PIX_SAT CH1_MEDIAN CH1_MEAN CH1_SD CH1_BKD_MEDIAN CH1_BKD_MEAN CH1_BKD_SD CH1_PERCNT_PIX_1SD CH1_PERCNT_PIX_2SD CH1_PERCNT_PIX_SAT RATIO_MEDIANS RATIO_MEANS MEDIAN_RATIOS MEAN_RATIOS RATIO_SD RGN_RATIO RGN_SQ_RATIO SPOT_PIXELS BKD_PIXELS LOG_RATIO CH2_MED_MINUS_BKD CH1_MED_MINUS_BKD CH2_MEAN_MINUS_BKD CH1_MEAN_MINUS_BKD FLAG NORM_RATIO VALUE MEAN_VALUE SD_VALUE NUM_REPLICATES
1 1870 23480 130 778 876 383 1095 1248 669 5 0 0 1042 1285 717 456 540 327 71 47 0 -0.541 -0.264 0.534 4.137 11.386 0.165 0.024 120 717 Error -317 586 -219 829 -10000 -10000 -10000 -7.049989459 2.211302966 31
2 2060 23470 100 1132 1277 593 1583 1621 570 10 3 0 454 585 550 608 680 399 6 2 0 2.929 13.304 2.699 4.932 10.102 0.837 0.334 80 440 1.55 -451 -154 -306 -23 -50 -10000 -10000 0.050408348 0.299594606 42
3 2230 23470 100 556 619 254 654 816 514 3 1 0 347 355 103 347 414 270 3 0 0 100000 -4.375 2.756 4.368 5.489 1.035 0.391 80 436 16.61 -98 0 -35 8 -50 -10000 -10000 -0.039622504 0 1
4 2410 23470 100 370 490 330 377 417 151 23 16 0 281 302 98 272 281 75 20 10 0 -0.778 3.767 2.735 6.381 15.406 1.353 0.26 80 440 Error -7 9 113 30 -50 -10000 -10000 0.050408348 0.299594606 42
5 2580 23460 100 454 509 208 357 369 86 55 28 0 359 367 83 267 279 80 53 16 0 1.054 1.52 1.168 2.427 4.532 0.721 0.184 80 383 0.076 97 92 152 100 -50 -10000 -10000 -0.094022236 0.272634061 8
6 2740 23460 120 3168 3084 1450 345 350 79 100 100 0 3053 3016 1589 268 276 80 100 98 0 1.014 0.997 1.002 1.134 0.648 0.94 0.939 120 500 0.02 2823 2785 2739 2748 0 1.047695411 0.067219353 -0.142936285 0.157994632 15
7 2910 23450 110 856 876 249 350 359 84 100 95 0 635 650 258 272 275 56 97 92 0 1.394 1.392 1.473 1.854 1.851 1.027 0.687 80 482 0.479 506 363 526 378 0 1.414699408 0.500495546 0.115757399 0.198824702 15
8 3110 23450 100 385 448 191 361 366 85 36 21 0 268 286 83 272 276 64 21 7 0 -6 6.214 1.996 5.246 11.7 0.46 0.066 80 427 Error 24 -4 87 14 -50 -10000 -10000 0.050408348 0.299594606 42
9 3260 23440 110 2822 3212 1446 377 410 266 100 100 0 3138 3410 1820 278 299 173 100 100 0 0.855 0.905 0.892 1.017 0.431 0.788 0.921 80 420 -0.226 2445 2860 2835 3132 0 0.885018832 -0.17621994 -0.30321345 0.12699351 2
10 3450 23440 120 1371 1312 347 363 387 233 95 89 0 849 838 232 268 286 148 94 86 0 1.735 1.665 1.67 2.051 3.057 1.477 0.829 120 557 0.795 1008 581 949 570 0 1.762094006 0.817290892 0.879662256 0.062371364 2
11 3630 23430 100 410 474 229 350 372 159 26 13 0 299 307 96 260 300 384 0 0 0 1.538 2.638 1.659 4.157 8.177 0.721 0.14 80 423 0.621 60 39 124 47 -50 -10000 -10000 0.050408348 0.299594606 42
12 3810 23430 100 389 476 252 337 354 118 35 18 0 288 311 109 259 282 262 6 1 0 1.793 2.673 1.668 5.481 14.659 0.346 0.318 80 427 0.842 52 29 139 52 -50 -10000 -10000 0.050408348 0.299594606 42
13 3990 23420 100 415 503 271 353 369 91 43 25 0 303 325 100 262 271 74 31 16 0 1.512 2.381 1.943 4.766 12.434 0.808 0.201 80 427 0.597 62 41 150 63 -50 -10000 -10000 0.050408348 0.299594606 42
14 4160 23420 100 381 459 219 366 375 81 35 18 0 287 304 97 275 284 105 16 3 0 1.25 3.207 1.447 3.43 5.797 0.358 0.099 80 444 0.322 15 12 93 29 -50 -10000 -10000 0.050408348 0.299594606 42
15 4340 23410 100 451 499 173 410 449 171 26 8 0 344 360 95 289 309 144 18 3 0 0.745 1.254 1.527 4.048 7.045 0.601 0.113 80 437 -0.424 41 55 89 71 -50 -10000 -10000 -0.288026949 0 1
16 4510 23410 100 445 511 192 450 489 206 20 7 0 335 342 113 305 319 118 12 3 0 -0.167 1.649 2.177 3.851 6.096 0.8 0.173 80 455 Error -5 30 61 37 -50 -10000 -10000 -0.007623701 0.204692782 7
17 4690 23400 100 393 458 271 416 442 135 18 8 0 302 336 206 292 298 93 21 7 0 -2.3 0.955 1.647 3.306 4.948 0.696 0.289 80 430 Error -23 10 42 44 -50 -10000 -10000 0.149802367 0.203000366 4
18 4860 23400 100 413 474 178 387 410 115 26 17 0 307 313 72 289 294 82 21 3 0 1.444 3.625 1.739 3.647 4.73 0.503 0.061 80 427 0.531 26 18 87 24 -50 -10000 -10000 0.268597601 0.33005378 5
19 5040 23390 120 971 967 271 360 369 97 95 92 0 1019 1048 435 272 278 80 92 91 0 0.818 0.782 0.792 1.005 0.975 0.654 0.742 120 584 -0.29 611 747 607 776 0 0.851610861 -0.231733745 -0.246385598 0.260446287 15
20 5230 23360 120 2831 2884 1484 354 363 97 99 96 0 2099 2127 1151 269 278 140 96 94 0 1.354 1.362 1.352 1.464 0.566 1.254 0.894 120 504 0.437 2477 1830 2530 1858 0 1.39426321 0.47950294 0.307262658 0.115869771 18

Total number of rows: 5760

Table truncated, full table size 1176 Kbytes.




Supplementary data files not provided
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