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Sample GSM1501 Query DataSets for GSM1501
Status Public on Apr 18, 2002
Title prp4-TempShift-0mins_3
Sample type RNA
 
Channel 1
Source name SRY4-1a (prp4-1) @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name SS330 (wt) @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Prp4-1 and wt strains were grown at 26deg to A600 of 1.0, then an equal volume of 48deg media was added to bring the temperature to 37deg. Both strains were allowed to grow at 37deg and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature.
Lot batch = Slide4-1046
 
Submission date Mar 19, 2002
Last update date May 27, 2005
Contact name Manuel Ares
E-mail(s) ares@biology.ucsc.edu
Phone 831 459-4628
Organization name UC Santa Cruz
Street address
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platform ID GPL108
Series (1)
GSE34 Prp4-1 temperature shift

Data table header descriptions
ID_REF
X_COORD X-coordinate of spot
Y_COORD Y-coordinate of spot
SPOT_SIZE Size of the spot (diameter, in microns)
CH2_MEDIAN Channel 2 (Cy5/635) median pixel intensity - feature
CH2_MEAN Channel 2 (Cy5/635) mean pixel intensity - feature
CH2_SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
CH2_BKD_MEDIAN Channel 2 (Cy5/635) median background intensity
CH2_BKD_MEAN Channel 2 (Cy5/635) mean background intensity
CH2_BKD_SD Channel 2 (Cy5/635) standard deviation of background
CH2_PERCNT_PIX_1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
CH2_PERCNT_PIX_2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
CH2_PERCNT_PIX_SAT Channel 2 (Cy5/635) percent of pixels saturated
CH1_MEDIAN Channel 1 (Cy3/532) median pixel intensity - feature
CH1_MEAN Channel 1 (Cy3/532) mean pixel intensity - feature
CH1_SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
CH1_BKD_MEDIAN Channel 1 (Cy3/532) median background intensity
CH1_BKD_MEAN Channel 1 (Cy3/532) mean background intensity
CH1_BKD_SD Channel 1 (Cy3/532) standard deviation of background
CH1_PERCNT_PIX_1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
CH1_PERCNT_PIX_2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
CH1_PERCNT_PIX_SAT Channel 1 (Cy3/532) percent of pixels saturated
RATIO_MEDIANS Ratio of medians (Ch2/Ch1)
RATIO_MEANS Ratio of means (Ch2/Ch1)
MEDIAN_RATIOS Median of ratios
MEAN_RATIOS Mean of ratios
RATIO_SD Ratio standard deviation
RGN_RATIO Regression ratio
RGN_SQ_RATIO Regression square ratio
SPOT_PIXELS Number of feature pixels
BKD_PIXELS Number of background pixels
LOG_RATIO Log ratio (base 2)
CH2_MED_MINUS_BKD Channel 2 (Cy5/635) background subtracted median feature intensity
CH1_MED_MINUS_BKD Channel 1 (Cy3/532) background subtracted median feature intensity
CH2_MEAN_MINUS_BKD Channel 2 (Cy5/635) background subtracted mean feature intensity
CH1_MEAN_MINUS_BKD Channel 1 (Cy3/532) background subtracted mean feature intensity
FLAG User defined flag (default=0)
NORM_RATIO Normalized ratio of medians
VALUE Normalized log ratio (base 2)
MEAN_VALUE Average normalized log ratio for replicate spots
SD_VALUE Standard deviation of average normalized log ratio
NUM_REPLICATES Number of replicate spots used in average normalized log ratio

Data table
ID_REF X_COORD Y_COORD SPOT_SIZE CH2_MEDIAN CH2_MEAN CH2_SD CH2_BKD_MEDIAN CH2_BKD_MEAN CH2_BKD_SD CH2_PERCNT_PIX_1SD CH2_PERCNT_PIX_2SD CH2_PERCNT_PIX_SAT CH1_MEDIAN CH1_MEAN CH1_SD CH1_BKD_MEDIAN CH1_BKD_MEAN CH1_BKD_SD CH1_PERCNT_PIX_1SD CH1_PERCNT_PIX_2SD CH1_PERCNT_PIX_SAT RATIO_MEDIANS RATIO_MEANS MEDIAN_RATIOS MEAN_RATIOS RATIO_SD RGN_RATIO RGN_SQ_RATIO SPOT_PIXELS BKD_PIXELS LOG_RATIO CH2_MED_MINUS_BKD CH1_MED_MINUS_BKD CH2_MEAN_MINUS_BKD CH1_MEAN_MINUS_BKD FLAG NORM_RATIO VALUE MEAN_VALUE SD_VALUE NUM_REPLICATES
1 2830 24780 110 107 184 262 98 99 13 36 27 0 255 764 1105 41 42 9 100 100 0 0.042 0.119 0.092 0.125 4.579 0.025 0.013 80 512 -4.572 9 214 86 723 0 0.155382334 -2.686105607 -3.674316578 1.815987024 30
2 3000 24780 100 105 168 198 93 93 13 43 30 0 54 62 29 41 42 9 58 36 0 0.923 3.571 2 1.749 4.241 7.365 0.573 80 391 -0.115 12 13 75 21 -50 -10000 -10000 0.284741777 0.460494403 21
3 3180 24770 120 102 113 61 87 93 121 3 2 0 76 79 19 40 46 119 0 0 0 0.417 0.667 0.467 0.464 2.705 0.715 0.93 120 516 -1.263 15 36 26 39 0 -10000 -10000 -0.192882552 0 1
4 3350 24770 100 100 154 235 80 88 136 10 5 0 56 67 43 40 49 135 1 1 0 1.25 2.741 1.333 1.409 3.137 5.729 0.512 80 408 0.322 20 16 74 27 -50 -10000 -10000 0.284741777 0.460494403 21
5 3540 24770 120 205 217 62 74 77 31 100 100 0 190 192 35 41 44 23 100 100 0 0.879 0.947 0.904 0.923 1.364 1 0.737 120 513 -0.186 131 149 143 151 0 0.950574279 -0.07312873 -0.062686735 0.318724938 14
6 3710 24760 130 25823 24892 3173 74 83 87 100 100 0 27818 26968 3339 42 49 89 100 100 0 0.927 0.922 0.927 0.921 1.101 0.919 0.986 120 478 -0.109 25749 27776 24818 26926 0 0.982951557 -0.024807777 -0.296586624 0.27744305 16
7 3880 24760 120 19938 19555 1889 76 87 89 100 100 0 31258 30217 3118 41 49 91 100 100 0 0.636 0.646 0.643 0.646 1.073 0.637 0.985 120 463 -0.652 19862 31217 19479 30176 0 0.674902683 -0.567248607 -0.706116119 0.178281666 16
8 4050 24760 100 101 165 236 71 74 19 61 40 0 81 104 62 40 43 13 85 61 0 0.732 1.469 0.667 0.869 2.976 4.947 0.32 80 391 -0.451 30 41 94 64 -50 -10000 -10000 0.284741777 0.460494403 21
9 4240 24750 130 9917 9150 2604 69 72 32 100 100 0 8578 8010 2258 40 42 17 100 100 0 1.153 1.139 1.143 1.137 1.11 1.143 0.988 120 602 0.206 9848 8538 9081 7970 0 1.222457872 0.289784749 0.208707317 0.081077432 2
10 4420 24750 130 2264 2263 298 63 67 34 100 100 0 1581 1589 232 40 41 17 100 100 0 1.428 1.42 1.413 1.423 1.084 1.405 0.98 120 555 0.514 2201 1541 2200 1549 0 1.507107083 0.591781927 -0.322083472 0.913865399 2
11 4580 24750 100 88 264 574 63 64 10 73 55 0 48 72 70 39 40 6 70 45 0 2.778 6.091 2.25 2.578 2.806 8.166 0.941 80 353 1.474 25 9 201 33 -50 -10000 -10000 0.284741777 0.460494403 21
12 4750 24750 100 79 232 457 66 67 11 53 40 0 50 68 60 40 40 6 63 45 0 1.3 5.929 2.405 2.023 3.618 7.954 0.859 80 394 0.379 13 10 166 28 -50 -10000 -10000 0.284741777 0.460494403 21
13 4930 24750 100 79 213 434 65 65 10 58 41 0 48 64 57 39 40 6 57 42 0 1.556 5.92 2.444 2.159 3.702 7.879 0.852 80 428 0.637 14 9 148 25 -50 -10000 -10000 0.284741777 0.460494403 21
14 5110 24740 100 75 278 698 64 65 12 43 32 0 50 77 101 39 40 6 65 43 0 1 5.632 2.388 2.206 3.499 6.906 0.962 80 421 0 11 11 214 38 -50 -10000 -10000 0.284741777 0.460494403 21
15 5300 24740 130 2870 2864 269 62 63 12 100 100 0 1701 1673 178 39 40 12 100 100 0 1.69 1.715 1.708 1.724 1.114 1.711 0.984 120 564 0.757 2808 1662 2802 1634 0 1.780928104 0.832629276 0.698359472 0.134269804 2
16 5470 24740 130 170 188 93 57 58 10 99 99 0 141 139 32 38 39 12 98 97 0 1.097 1.297 1.138 1.242 1.49 1.733 0.682 120 502 0.134 113 103 131 101 0 1.110346758 0.151010296 0.303539037 0.266949494 13
17 5650 24730 120 80 94 59 56 57 8 87 68 0 74 75 18 37 38 6 98 95 0 0.649 1 0.683 0.687 2.373 2.551 0.35 120 458 -0.624 24 37 38 38 0 0.865515042 -0.208369202 0.221763298 0.290404515 10
18 5820 24730 120 79 108 119 57 57 8 87 67 0 70 74 22 37 38 6 100 97 0 0.667 1.378 0.687 0.719 2.418 4.668 0.47 120 430 -0.585 22 33 51 37 0 0.895327227 -0.159513036 0.19036501 0.324733047 15
19 5990 24730 130 447 448 113 55 56 10 100 100 0 280 276 44 38 38 7 99 99 0 1.62 1.651 1.606 1.636 1.23 1.753 0.905 120 504 0.696 392 242 393 238 0 1.706349382 0.770913074 0.52404303 0.213648232 19
20 6170 24730 130 5904 5828 927 55 55 9 100 100 0 15477 15294 2514 38 39 7 100 100 0 0.379 0.378 0.376 0.38 1.082 0.375 0.988 120 500 -1.4 5849 15439 5773 15256 0 0.402544588 -1.312779502 -1.256263432 0.173630316 20

Total number of rows: 5760

Table truncated, full table size 1134 Kbytes.




Supplementary data files not provided
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