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Sample GSM1504 Query DataSets for GSM1504
Status Public on Apr 16, 2002
Title prp4-TempShift-15mins_5
Sample type RNA
 
Channel 1
Source name SS330 (wt) @ 37 deg for 15mins
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name SRY4-1a (prp4-1) @ 37 deg for 15 mins
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Prp4-1 and wt strains were grown at 26deg to A600 of 1.0, then an equal volume of 48deg media was added to bring the temperature to 37deg. Both strains were allowed to grow at 37deg and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature.
Lot batch = Slide4-34
 
Submission date Mar 19, 2002
Last update date May 27, 2005
Contact name Manuel Ares
E-mail(s) ares@biology.ucsc.edu
Phone 831 459-4628
Organization name UC Santa Cruz
Street address
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platform ID GPL108
Series (1)
GSE34 Prp4-1 temperature shift

Data table header descriptions
ID_REF
X_COORD X-coordinate of spot
Y_COORD Y-coordinate of spot
SPOT_SIZE Size of the spot (diameter, in microns)
CH2_MEDIAN Channel 2 (Cy5/635) median pixel intensity - feature
CH2_MEAN Channel 2 (Cy5/635) mean pixel intensity - feature
CH2_SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
CH2_BKD_MEDIAN Channel 2 (Cy5/635) median background intensity
CH2_BKD_MEAN Channel 2 (Cy5/635) mean background intensity
CH2_BKD_SD Channel 2 (Cy5/635) standard deviation of background
CH2_PERCNT_PIX_1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
CH2_PERCNT_PIX_2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
CH2_PERCNT_PIX_SAT Channel 2 (Cy5/635) percent of pixels saturated
CH1_MEDIAN Channel 1 (Cy3/532) median pixel intensity - feature
CH1_MEAN Channel 1 (Cy3/532) mean pixel intensity - feature
CH1_SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
CH1_BKD_MEDIAN Channel 1 (Cy3/532) median background intensity
CH1_BKD_MEAN Channel 1 (Cy3/532) mean background intensity
CH1_BKD_SD Channel 1 (Cy3/532) standard deviation of background
CH1_PERCNT_PIX_1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
CH1_PERCNT_PIX_2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
CH1_PERCNT_PIX_SAT Channel 1 (Cy3/532) percent of pixels saturated
RATIO_MEDIANS Ratio of medians (Ch2/Ch1)
RATIO_MEANS Ratio of means (Ch2/Ch1)
MEDIAN_RATIOS Median of ratios
MEAN_RATIOS Mean of ratios
RATIO_SD Ratio standard deviation
RGN_RATIO Regression ratio
RGN_SQ_RATIO Regression square ratio
SPOT_PIXELS Number of feature pixels
BKD_PIXELS Number of background pixels
LOG_RATIO Log ratio (base 2)
CH2_MED_MINUS_BKD Channel 2 (Cy5/635) background subtracted median feature intensity
CH1_MED_MINUS_BKD Channel 1 (Cy3/532) background subtracted median feature intensity
CH2_MEAN_MINUS_BKD Channel 2 (Cy5/635) background subtracted mean feature intensity
CH1_MEAN_MINUS_BKD Channel 1 (Cy3/532) background subtracted mean feature intensity
FLAG User defined flag (default=0)
NORM_RATIO Normalized ratio of medians
VALUE Normalized log ratio (base 2)
MEAN_VALUE Average normalized log ratio for replicate spots
SD_VALUE Standard deviation of average normalized log ratio
NUM_REPLICATES Number of replicate spots used in average normalized log ratio

Data table
ID_REF X_COORD Y_COORD SPOT_SIZE CH2_MEDIAN CH2_MEAN CH2_SD CH2_BKD_MEDIAN CH2_BKD_MEAN CH2_BKD_SD CH2_PERCNT_PIX_1SD CH2_PERCNT_PIX_2SD CH2_PERCNT_PIX_SAT CH1_MEDIAN CH1_MEAN CH1_SD CH1_BKD_MEDIAN CH1_BKD_MEAN CH1_BKD_SD CH1_PERCNT_PIX_1SD CH1_PERCNT_PIX_2SD CH1_PERCNT_PIX_SAT RATIO_MEDIANS RATIO_MEANS MEDIAN_RATIOS MEAN_RATIOS RATIO_SD RGN_RATIO RGN_SQ_RATIO SPOT_PIXELS BKD_PIXELS LOG_RATIO CH2_MED_MINUS_BKD CH1_MED_MINUS_BKD CH2_MEAN_MINUS_BKD CH1_MEAN_MINUS_BKD FLAG NORM_RATIO VALUE MEAN_VALUE SD_VALUE NUM_REPLICATES
1 2400 22640 120 576 657 369 576 608 268 11 6 0 1708 1945 1228 315 340 236 94 82 0 0 0.05 0.157 0.506 1.069 0.063 0.032 120 613 Error 0 1393 81 1630 0 0.018310626 -5.771175108 -7.244413546 2.337151297 31
2 2580 22630 100 599 686 492 570 678 871 3 1 0 341 380 217 319 384 492 3 1 0 1.318 1.902 2.137 3.768 5.267 1.835 0.841 80 424 0.399 29 22 116 61 -50 -10000 -10000 0.284600951 0.279479298 16
3 2750 22630 100 549 587 201 549 654 926 1 0 0 340 360 94 322 388 631 0 0 0 0 1 1.607 5.301 12.35 0.864 0.904 80 436 Error 0 18 38 38 -50 -10000 -10000 -10000 -10000 -10000
4 2930 22630 100 585 694 355 542 588 363 18 7 0 329 388 309 313 324 171 13 5 0 2.688 2.027 2.759 4.041 4.418 0.721 0.163 80 440 1.426 43 16 152 75 -50 -10000 -10000 0.284600951 0.279479298 16
5 3100 22630 100 608 669 218 540 579 370 13 2 0 413 417 97 316 328 174 18 2 0 0.701 1.277 1.319 3.085 4.974 0.671 0.097 80 419 -0.512 68 97 129 101 -50 -10000 -10000 0.218788358 0.169288113 6
6 3280 22640 120 5974 5593 2198 545 557 148 100 99 0 5268 5082 2152 318 326 98 100 98 0 1.097 1.06 1.071 1.183 0.749 1.027 0.951 120 520 0.133 5429 4950 5048 4764 0 1.1385745 0.187228694 -0.071843148 0.148819309 15
7 3460 22640 120 1556 1549 765 562 602 372 86 64 0 880 992 786 316 359 422 70 18 0 1.762 1.46 1.629 1.96 1.96 1.002 0.778 120 503 0.818 994 564 987 676 0 1.79679402 0.845425031 0.500933498 0.154581612 15
8 3630 22630 100 595 694 350 545 588 393 13 6 0 320 350 150 314 372 492 1 0 0 8.333 4.139 2.762 6.725 18.593 0.66 0.648 80 400 3.059 50 6 149 36 -50 -10000 -10000 0.284600951 0.279479298 16
9 3810 22640 120 2408 2706 1132 539 556 220 98 97 0 1831 1934 853 318 336 188 98 95 0 1.235 1.341 1.31 1.538 1.147 1.219 0.857 120 503 0.305 1869 1513 2167 1616 0 1.27897791 0.354991347 0.302527482 0.052463864 2
10 3990 22640 110 2187 2172 401 545 617 678 100 77 0 884 918 195 324 380 733 26 0 0 2.932 2.739 2.727 2.93 0.897 0.999 0.711 80 442 1.552 1642 560 1627 594 0 2.959505409 1.565356094 1.602095675 0.036739582 2
11 4150 22630 100 637 774 386 511 547 185 41 26 0 330 354 104 320 332 123 20 5 0 12.6 7.735 2.603 6.168 12.732 1.209 0.155 80 439 3.655 126 10 263 34 -50 -10000 -10000 0.284600951 0.279479298 16
12 4330 22620 100 532 597 278 551 616 333 8 5 0 305 324 84 323 356 278 1 0 0 1.056 46 1.88 3.441 4.154 0.386 0.26 80 441 0.078 -19 -18 46 1 -50 -10000 -10000 0.284600951 0.279479298 16
13 4500 22620 100 726 809 355 632 761 435 22 5 0 378 410 149 344 382 240 12 1 0 2.765 2.682 2.207 3.836 4.33 1.57 0.304 80 441 1.467 94 34 177 66 -50 -10000 -10000 0.284600951 0.279479298 16
14 4680 22620 100 507 555 213 515 545 163 16 11 0 318 323 89 301 319 122 10 2 0 -0.471 1.818 2.201 6.859 14.535 0.326 0.075 80 472 Error -8 17 40 22 -50 -10000 -10000 0.284600951 0.279479298 16
15 4870 22630 90 698 725 203 493 511 131 65 38 0 397 408 115 309 327 128 40 13 0 2.33 2.343 1.55 2.714 3.506 0.506 0.11 52 369 1.22 205 88 232 99 0 2.113921213 1.079921608 0.804057818 0.27586379 2
16 5030 22620 100 547 582 157 496 520 195 25 5 0 322 336 99 310 322 137 11 2 0 4.25 3.308 1.975 4.52 8.84 0.477 0.116 80 443 2.087 51 12 86 26 -50 -10000 -10000 0.470294344 0.186201979 2
17 5200 22620 100 537 641 384 498 505 111 30 20 0 338 352 92 312 319 84 25 10 0 1.5 3.575 1.189 3.459 7.338 0.181 0.005 80 442 0.585 39 26 143 40 -50 -10000 -10000 0.194568157 0.017583639 2
18 5380 22620 100 576 604 212 492 503 111 38 21 0 340 358 113 315 323 86 30 12 0 3.36 2.605 1.803 4.083 8.7 0.446 0.079 80 449 1.748 84 25 112 43 -50 -10000 -10000 0.260911089 0.215431582 2
19 5570 22630 120 795 852 302 502 514 115 87 61 0 493 510 118 308 321 80 85 60 0 1.584 1.733 1.532 2.202 2.166 1.022 0.286 120 575 0.663 293 185 350 202 0 1.572704863 0.653247957 0.380337854 0.171912606 14
20 5760 22610 120 4923 5002 2732 502 507 109 99 99 0 2665 2702 1526 302 312 79 97 97 0 1.871 1.875 1.884 2.169 1.231 1.781 0.934 120 526 0.904 4421 2363 4500 2400 0 1.933635186 0.951315631 0.767693379 0.104079863 19

Total number of rows: 5761

Table truncated, full table size 1166 Kbytes.




Supplementary data files not provided
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