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Sample GSM1510 Query DataSets for GSM1510
Status Public on Apr 18, 2002
Title prp4-TempShift-120mins_5
Sample type RNA
 
Channel 1
Source name SS330 (wt) @ 37 deg for 120 mins
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name SRY4-1a (prp4-1) @ 37 deg for 120 mins
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Prp4-1 and wt strains were grown at 26deg to A600 of 1.0, then an equal volume of 48deg media was added to bring the temperature to 37deg. Both strains were allowed to grow at 37deg and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature.
Lot batch = Slide4-37
 
Submission date Mar 19, 2002
Last update date May 27, 2005
Contact name Manuel Ares
E-mail(s) ares@biology.ucsc.edu
Phone 831 459-4628
Organization name UC Santa Cruz
Street address
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platform ID GPL108
Series (1)
GSE34 Prp4-1 temperature shift

Data table header descriptions
ID_REF
X_COORD X-coordinate of spot
Y_COORD Y-coordinate of spot
SPOT_SIZE Size of the spot (diameter, in microns)
CH2_MEDIAN Channel 2 (Cy5/635) median pixel intensity - feature
CH2_MEAN Channel 2 (Cy5/635) mean pixel intensity - feature
CH2_SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
CH2_BKD_MEDIAN Channel 2 (Cy5/635) median background intensity
CH2_BKD_MEAN Channel 2 (Cy5/635) mean background intensity
CH2_BKD_SD Channel 2 (Cy5/635) standard deviation of background
CH2_PERCNT_PIX_1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
CH2_PERCNT_PIX_2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
CH2_PERCNT_PIX_SAT Channel 2 (Cy5/635) percent of pixels saturated
CH1_MEDIAN Channel 1 (Cy3/532) median pixel intensity - feature
CH1_MEAN Channel 1 (Cy3/532) mean pixel intensity - feature
CH1_SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
CH1_BKD_MEDIAN Channel 1 (Cy3/532) median background intensity
CH1_BKD_MEAN Channel 1 (Cy3/532) mean background intensity
CH1_BKD_SD Channel 1 (Cy3/532) standard deviation of background
CH1_PERCNT_PIX_1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
CH1_PERCNT_PIX_2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
CH1_PERCNT_PIX_SAT Channel 1 (Cy3/532) percent of pixels saturated
RATIO_MEDIANS Ratio of medians (Ch2/Ch1)
RATIO_MEANS Ratio of means (Ch2/Ch1)
MEDIAN_RATIOS Median of ratios
MEAN_RATIOS Mean of ratios
RATIO_SD Ratio standard deviation
RGN_RATIO Regression ratio
RGN_SQ_RATIO Regression square ratio
SPOT_PIXELS Number of feature pixels
BKD_PIXELS Number of background pixels
LOG_RATIO Log ratio (base 2)
CH2_MED_MINUS_BKD Channel 2 (Cy5/635) background subtracted median feature intensity
CH1_MED_MINUS_BKD Channel 1 (Cy3/532) background subtracted median feature intensity
CH2_MEAN_MINUS_BKD Channel 2 (Cy5/635) background subtracted mean feature intensity
CH1_MEAN_MINUS_BKD Channel 1 (Cy3/532) background subtracted mean feature intensity
FLAG User defined flag (default=0)
NORM_RATIO Normalized ratio of medians
VALUE Normalized log ratio (base 2)
MEAN_VALUE Average normalized log ratio for replicate spots
SD_VALUE Standard deviation of average normalized log ratio
NUM_REPLICATES Number of replicate spots used in average normalized log ratio

Data table
ID_REF X_COORD Y_COORD SPOT_SIZE CH2_MEDIAN CH2_MEAN CH2_SD CH2_BKD_MEDIAN CH2_BKD_MEAN CH2_BKD_SD CH2_PERCNT_PIX_1SD CH2_PERCNT_PIX_2SD CH2_PERCNT_PIX_SAT CH1_MEDIAN CH1_MEAN CH1_SD CH1_BKD_MEDIAN CH1_BKD_MEAN CH1_BKD_SD CH1_PERCNT_PIX_1SD CH1_PERCNT_PIX_2SD CH1_PERCNT_PIX_SAT RATIO_MEDIANS RATIO_MEANS MEDIAN_RATIOS MEAN_RATIOS RATIO_SD RGN_RATIO RGN_SQ_RATIO SPOT_PIXELS BKD_PIXELS LOG_RATIO CH2_MED_MINUS_BKD CH1_MED_MINUS_BKD CH2_MEAN_MINUS_BKD CH1_MEAN_MINUS_BKD FLAG NORM_RATIO VALUE MEAN_VALUE SD_VALUE NUM_REPLICATES
1 1790 22960 130 466 561 291 413 425 99 33 20 0 1534 1584 883 254 259 57 98 97 0 0.041 0.111 0.127 0.541 0.873 0.034 0.02 120 712 -4.594 53 1280 148 1330 0 0.073090323 -3.774175777 -6.746795179 2.381475739 32
2 1950 22970 100 473 663 449 417 426 93 42 35 0 274 353 177 263 266 59 37 28 0 5.091 2.733 2.071 5.569 11.9 1.862 0.588 80 374 2.348 56 11 246 90 -50 -10000 -10000 -0.32737014 0.72350537 33
3 2120 22970 100 516 605 296 426 427 86 50 33 0 351 370 101 264 269 55 63 38 0 1.034 1.689 1.925 3.826 7.599 1.146 0.252 80 434 0.049 90 87 179 106 -50 -10000 -10000 -0.822752867 0 1
4 2300 22970 100 505 762 579 421 431 89 48 40 0 283 345 197 259 268 60 31 22 0 3.5 3.965 2.686 6.839 12.878 2.228 0.584 80 444 1.807 84 24 341 86 -50 -10000 -10000 -0.32737014 0.72350537 33
5 2470 22970 100 515 709 434 418 426 87 52 40 0 363 411 168 249 256 51 77 56 0 0.851 1.796 1.946 3.687 7.572 1.458 0.44 80 411 -0.233 97 114 291 162 -50 -10000 -10000 -0.362958236 0.588071257 8
6 2660 22960 120 6625 6434 2421 420 426 91 100 100 0 5159 5143 2445 252 258 50 100 100 0 1.265 1.23 1.274 1.337 0.605 1.119 0.907 120 542 0.339 6205 4907 6014 4891 0 1.54468799 0.627315459 0.60138257 0.169997887 15
7 2830 22960 110 1358 1446 408 422 429 95 100 100 0 771 789 194 255 261 60 100 100 0 1.814 1.918 1.857 2.006 0.688 1.693 0.795 80 450 0.859 936 516 1024 534 0 2.172210525 1.119163932 0.806497182 0.297288958 15
8 3000 22970 100 488 807 705 413 418 90 43 30 0 279 383 259 248 248 54 45 31 0 2.419 2.919 2.336 4.378 8.676 2.203 0.704 80 411 1.275 75 31 394 135 -50 -10000 -10000 -0.32737014 0.72350537 33
9 3190 22960 120 670 772 399 410 411 89 87 67 0 5069 5329 2204 247 249 50 99 99 0 0.054 0.071 0.048 0.103 0.139 0.05 0.207 120 510 -4.213 260 4822 362 5082 0 0.071903103 -3.797802163 -3.882739074 0.084936911 2
10 3360 22950 110 2951 3112 728 414 429 165 100 100 0 1600 1602 326 249 259 82 100 100 0 1.878 1.994 2.002 2.025 0.387 1.956 0.946 80 454 0.909 2537 1351 2698 1353 0 2.276860813 1.187046101 0.922596958 0.264449143 2
11 3520 22970 100 480 673 455 409 428 166 38 26 0 267 329 158 251 263 81 32 18 0 4.438 3.385 2.59 3.734 4.02 1.826 0.464 80 401 2.15 71 16 264 78 -50 -10000 -10000 -0.32737014 0.72350537 33
12 3700 22970 100 539 711 462 427 437 91 53 36 0 289 343 165 255 262 50 43 31 0 3.294 3.227 2.789 5.499 10.561 1.966 0.536 80 413 1.72 112 34 284 88 -50 -10000 -10000 -0.32737014 0.72350537 33
13 3870 22970 100 469 685 463 449 455 94 36 28 0 287 349 175 271 271 53 33 27 0 1.25 3.026 2.086 2.813 2.702 1.756 0.483 80 432 0.322 20 16 236 78 -50 -10000 -10000 -0.32737014 0.72350537 33
14 4050 22970 100 546 631 291 449 453 94 50 28 0 295 326 117 278 281 57 37 17 0 5.706 3.792 2.218 3.46 3.437 1.448 0.37 80 427 2.512 97 17 182 48 -50 -10000 -10000 -0.32737014 0.72350537 33
15 4220 22970 100 490 592 310 447 445 89 36 21 0 412 448 144 282 285 57 81 61 0 0.331 0.873 0.751 1.213 1.542 0.75 0.224 80 432 -1.596 43 130 145 166 -50 -10000 -10000 -0.136754046 0 1
16 4400 22970 100 469 597 348 426 430 86 40 26 0 319 335 122 271 274 55 43 21 0 0.896 2.672 1.758 2.565 2.418 1.613 0.419 80 444 -0.159 43 48 171 64 -50 -10000 -10000 -0.336427804 0.419877794 3
17 4570 22970 100 434 529 247 394 404 87 41 23 0 264 308 127 256 257 51 40 21 0 5 2.596 2.1 3.667 4.968 1.082 0.299 80 433 2.322 40 8 135 52 -50 -10000 -10000 -0.173616152 0.150496194 3
18 4750 22970 100 416 536 316 372 381 82 38 31 0 262 292 109 233 238 49 33 27 0 1.517 2.78 2.516 5.146 8.144 1.486 0.34 80 444 0.601 44 29 164 59 -50 -10000 -10000 -0.05462166 0.603954622 6
19 4950 22950 100 489 656 467 377 382 92 53 35 0 569 639 310 245 248 50 88 78 0 0.346 0.708 0.675 1.181 1.454 0.702 0.42 80 456 -1.532 112 324 279 394 0 0.48003282 -1.058795048 -1.570336162 1.581832667 15
20 5130 22930 110 6358 6157 2752 409 413 106 100 100 0 3412 3551 1674 256 262 81 100 100 0 1.885 1.744 1.749 1.78 0.32 1.669 0.961 80 495 0.915 5949 3156 5748 3295 0 2.296557588 1.199472961 0.968261429 0.164987772 19

Total number of rows: 5761

Table truncated, full table size 1172 Kbytes.




Supplementary data files not provided

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