Data table header descriptions |
ID_REF |
|
X_COORD |
X-coordinate of spot |
Y_COORD |
Y-coordinate of spot |
SPOT_SIZE |
Size of the spot (diameter, in microns) |
CH2_MEDIAN |
Channel 2 (Cy5/635) median pixel intensity - feature |
CH2_MEAN |
Channel 2 (Cy5/635) mean pixel intensity - feature |
CH2_SD |
Channel 2 (Cy5/635) standard deviation of pixel intensities - feature |
CH2_BKD_MEDIAN |
Channel 2 (Cy5/635) median background intensity |
CH2_BKD_MEAN |
Channel 2 (Cy5/635) mean background intensity |
CH2_BKD_SD |
Channel 2 (Cy5/635) standard deviation of background |
CH2_PERCNT_PIX_1SD |
Channel 2 (Cy5/635) percent of pixels 1 StDev above background |
CH2_PERCNT_PIX_2SD |
Channel 2 (Cy5/635) percent of pixels 2 StDevs above background |
CH2_PERCNT_PIX_SAT |
Channel 2 (Cy5/635) percent of pixels saturated |
CH1_MEDIAN |
Channel 1 (Cy3/532) median pixel intensity - feature |
CH1_MEAN |
Channel 1 (Cy3/532) mean pixel intensity - feature |
CH1_SD |
Channel 1 (Cy3/532) standard deviation of pixel intensities - feature |
CH1_BKD_MEDIAN |
Channel 1 (Cy3/532) median background intensity |
CH1_BKD_MEAN |
Channel 1 (Cy3/532) mean background intensity |
CH1_BKD_SD |
Channel 1 (Cy3/532) standard deviation of background |
CH1_PERCNT_PIX_1SD |
Channel 1 (Cy3/532) percent of pixels 1 StDev above background |
CH1_PERCNT_PIX_2SD |
Channel 1 (Cy3/532) percent of pixels 2 StDevs above background |
CH1_PERCNT_PIX_SAT |
Channel 1 (Cy3/532) percent of pixels saturated |
RATIO_MEDIANS |
Ratio of medians (Ch2/Ch1) |
RATIO_MEANS |
Ratio of means (Ch2/Ch1) |
MEDIAN_RATIOS |
Median of ratios |
MEAN_RATIOS |
Mean of ratios |
RATIO_SD |
Ratio standard deviation |
RGN_RATIO |
Regression ratio |
RGN_SQ_RATIO |
Regression square ratio |
SPOT_PIXELS |
Number of feature pixels |
BKD_PIXELS |
Number of background pixels |
LOG_RATIO |
Log ratio (base 2) |
CH2_MED_MINUS_BKD |
Channel 2 (Cy5/635) background subtracted median feature intensity |
CH1_MED_MINUS_BKD |
Channel 1 (Cy3/532) background subtracted median feature intensity |
CH2_MEAN_MINUS_BKD |
Channel 2 (Cy5/635) background subtracted mean feature intensity |
CH1_MEAN_MINUS_BKD |
Channel 1 (Cy3/532) background subtracted mean feature intensity |
FLAG |
User defined flag (default=0) |
NORM_RATIO |
Normalized ratio of medians |
VALUE |
Normalized log ratio (base 2) |
MEAN_VALUE |
Average normalized log ratio for replicate spots |
SD_VALUE |
Standard deviation of average normalized log ratio |
NUM_REPLICATES |
Number of replicate spots used in average normalized log ratio |