|
| Status |
Public on Oct 17, 2014 |
| Title |
Human Male with Syndromic Intellectual disability_SNP-array |
| Sample type |
genomic |
| |
|
| Source name |
Peripheral blood DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extraction was performed with a commercial kit QIAamp ® DNA Mini Kit (QIAGEN). The extraction was performed automatically, using equipment QIAcube (QIAGEN) with the recommended protocol with minor modifications.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
| Data processing |
The array image was acquired using Affymetrix chromosome Analysis suite (ChAS version 2,0,1,2). Copy number values for individual SNPs were extracted and converted from CEL files.
|
| |
|
| Submission date |
Oct 16, 2014 |
| Last update date |
Oct 17, 2014 |
| Contact name |
Francisco Martinez |
| E-mail(s) |
martinez_fracas@gva.es
|
| Organization name |
Hospital Universitari i Politecnic La Fe
|
| Department |
Unidad de Genetica
|
| Street address |
Avenida Campanar 21
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46009 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE62438 |
Application of SNP-array for the detection of genomic rearrangements responsible for sindromic mental retardation of unknown cause |
| GSE62440 |
Application of CGH-array and SNP-array for the detection of genomic rearrangements responsible for sindromic mental retardation of unknown cause |
|
