GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1528448 Query DataSets for GSM1528448
Status Public on Mar 31, 2015
Title 23_20h_D
Sample type RNA
Source name mononuclear cells from peripheral blood
Organism Homo sapiens
Characteristics age (years): 67
gender: m
sample source: PB
karyotype: normal
igvh status: unmutated
tp53 mutation: MUT
treatment time: 20h
treatment: DMSO
Treatment protocol Primary CLL samples were cultivated using RPMI 1640 (Biochrom AG, Berlin, Germany), supplemented with 10% FCS (Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-Glutamin (Biochrom AG), and Penicillin/Streptomycin (GIBCO, Invitrogen Corporation, Grand Island, NY, USA). Prior to treatment, cells were stained with trypan blue (Sigma-Aldrich), counted, and diluted to a density of 1.0x106 cells/ml. Thawing of viably frozen samples followed the DSMZ (German Collection of microorganisms and cell lines, Braunschweig) guideline. Agents used to treat primary AML sample for 4 and 20 hours in vitro were DMSO (control/carrier; dimethyl sulfoxide; Sigma-Aldrich), and BV6 was synthesized at Genentech, Inc. (South San Francisco, CA, USA).
Extracted molecule total RNA
Extraction protocol The total RNA was purified with a TRIzol-based protocol.
Label Biotin
Label protocol For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
Hybridization protocol Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
Scan protocol Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
Description 23_20h_D
Data processing The data were analyzed with BRB ArrayTools using RMA. Intensity values were log2 transformed
Submission date Oct 20, 2014
Last update date Mar 31, 2015
Contact name Lars Bullinger
Phone +49-30-450-553111
Organization name Charité
Department Hematology, Oncology and Tumorimmunology
Street address Augustenburger Platz 1
City Berlin
ZIP/Postal code 13353
Country Germany
Platform ID GPL570
Series (1)
GSE62533 Inhibitor of apoptosis proteins as promising therapeutic targets in chronic lymphocytic leukemia

Data table header descriptions
VALUE RMA normalized data, log2 transformed

Data table
1007_s_at 9.226042747
1053_at 7.841062546
117_at 6.206427574
121_at 7.920425415
1255_g_at 3.251124382
1294_at 7.304114342
1316_at 7.556201458
1320_at 4.722914696
1405_i_at 8.726991653
1431_at 5.449664116
1438_at 5.645008087
1487_at 7.897689342
1494_f_at 6.291742325
1552256_a_at 6.259385109
1552257_a_at 8.163951874
1552258_at 5.451310158
1552261_at 4.069159508
1552263_at 8.070187569
1552264_a_at 8.38144207
1552266_at 2.838790417

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM1528448_K_20h_D.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap