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Sample GSM15335 Query DataSets for GSM15335
Status Public on Jan 10, 2005
Title AML patient M1282 (Repl. 1)
Sample type RNA
 
Channel 1
Source name Neutrophils from 7 healthy controls (RNA pooled)
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Neutrophils from AML patient
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description The patient RNA was compared to the normal RNA pool using a T7-based amplification method: 1 microgram of total RNA was reverse-transcribed using a T7-oligodT primer and then labelled aRNAs produced via in vitro transcription in the presence of either Cy3-UTP or Cy5-UTP. The labelled aRNAs were then purified and hybridized competitively to the microarray.
 
Submission date Jan 16, 2004
Last update date May 28, 2005
Contact name Andrea Pellagatti
E-mail(s) andreapellagatti@yahoo.co.uk
Phone 00441865222911
Organization name University of Oxford
Department NDCLS, RDM
Lab LLR Molecular Haematology Unit
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platform ID GPL334
Series (1)
GSE971 Myelodysplastic Syndromes (MDS) and Acute Myeloid Leukaemia (AML)

Data table header descriptions
ID_REF
CH1_NORM Channel 1 (Cy3, control) normalized intensity
CH2_NORM Channel 2 (Cy5, patient) normalized intensity
RATIO Ratio CH2/CH1
VALUE Log Ratio CH2/CH1

Data table
ID_REF CH1_NORM CH2_NORM RATIO VALUE
1 126.743698 62.68356095 0.49456945 -1.015754969
2 163.928574 111.0171067 0.677228527 -0.56228535
3 1389 1127.620508 0.81182182 -0.300764978
4 168.096634 122.0881116 0.726297182 -0.461368112
5 668.810913 811.8390401 1.213854356 0.279595331
6 144.205887 128.8083419 0.893225267 -0.162904033
7 608.789917 817.3948297 1.342655006 0.425088653
8 270.226898 365.3663718 1.352072553 0.43517257
9 211.394958 282.7943998 1.337753759 0.419812583
10 357.722687 654.330625 1.829156072 0.871178178
11 920.046204 1355.59285 1.473396493 0.559145714
12 12474.50879 24982.2791 2.002666359 1.00192209
13 1246.69751 2736.473472 2.194977892 1.134206409
14 295.941162 370.7599516 1.252816435 0.325175044
15 103.554619 84.13037788 0.81242516 -0.299693176
16 117.487396 70.40603751 0.599264601 -0.73873494
17 509.025208 3660.389864 7.190979555 2.846188308
18 729.697449 2042.985927 2.799771234 1.485308951
19 172.43277 467.1025906 2.708896868 1.437705469
20 620.168091 2488.635846 4.012840845 2.004623938

Total number of rows: 9984

Table truncated, full table size 509 Kbytes.




Supplementary data files not provided
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