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Sample GSM1536290 Query DataSets for GSM1536290
Status Public on Nov 04, 2015
Title SRRM2 siRNA 1 [gene-level]
Sample type RNA
 
Source name SRRM2 siRNA treatment of activated CD4 T cells
Organism Homo sapiens
Characteristics cell type: Primary CD4 T cells
pretreated with: SRRM2 siRNA for 24hrs
activated with: anti-CD3/CD26 activator beads for 24 h
passages: 7-8
Treatment protocol Primary CD4 T cells cultured were treated with siRNA 24 hours prior to treatment with anti-CD3/CD26 activator beads (Thermo) for an additional 24 hours.
Growth protocol Primary CD4 T cells cultured for two weeks in RPMI supplemented with 10% FBS, 30U/ml IL2, 100U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2
Extracted molecule total RNA
Extraction protocol Cell pellets were resuspended in TRIZOL reagent (Thermo) and the RNA was extracted according to manufacturer's protocol. RNA resuspended in nanopure water was purified over an Rneasy column (QIAGEN) and the polyA RNA was selected using oligo dT beads.
Label biotin
Label protocol Biotinylated cDNA was prepared using the Ambion WT Expression Kit (Ambion) according to the manufacturer's instructions. cDNA was framented and labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Standard Affymetrix 49 Format Hybridization with 15mg of fragmented cRNA
Scan protocol Affymetrix EukGE-WS2v4 on GCS 3000 7G
Description SAMPLE 19
Data processing Expression intenisties at the probe set (exon) and transcript (gene) level were made with Expression Console (Affymetrix).
Gene level expression intensities were used as input into the R Bioconductor package limma for identification of differentially expressed genes (Ctrl siRNA vs. Specific siRNA)
Exon level expression intensitied were used as input into Alt-Analyze (Emig, et al) to identify differentially spliced exons (Ctrl siRNA vs. Specific siRNA)
The Series supplementary 'TotalRNA_HTA2_all_siRNA_100414.xslx' file has multiple worksheets containing: 1) Differentially expressed genes in ctrl siRNA vs. U2AF1 siRNA; 2)Differntially spliced genes in ctrl siRNA vs. U2AF1 siRNA; 3)Differentially spliced exons in ctrl siRNA vs. U2AF1 siRNA; 4)Differentially expressed genes in ctrl siRNA vs. SRRM2 siRNA; 5)Differntially spliced genes in ctrl siRNA vs. SRRM2 siRNA; 6)Differentially spliced exons in ctrl siRNA vs. SRRM2 siRNA; 7)Differentially expressed genes in ctrl siRNA vs. SYNCRIP siRNA; 8)Differntially spliced genes in ctrl siRNA vs. SYNCRIP siRNA; 9)Differentially spliced exons in ctrl siRNA vs. SYNCRIP siRNA; 10)Differentially expressed genes in ctrl siRNA vs. ILF2 siRNA; 11)Differntially spliced genes in ctrl siRNA vs. ILF2 siRNA; 12)Differentially spliced exons in ctrl siRNA vs. ILF2 siRNA
probe group file: HuEx-1_0-st-v2.r2.pgf
meta-probeset file: HuEx-1_0-st-v2.r2.dt1.hg18.full.mps
 
Submission date Nov 03, 2014
Last update date Nov 04, 2015
Contact name Thomas C Whisenant
E-mail(s) thomas.whisenant@gmail.com
Organization name The Scripps Research Institute
Department Molecular and Experimental Medicine
Lab Salomon
Street address 10550 North Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL17586
Series (2)
GSE62921 The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells [total RNA-array]
GSE62923 The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells

Data table header descriptions
ID_REF
VALUE Normalized, log-transformed RMA values

Data table
ID_REF VALUE
TC01000001.hg.1 7.985759
TC01000002.hg.1 7.059107
TC01000003.hg.1 4.39612
TC01000004.hg.1 4.036314
TC01000005.hg.1 9.601975
TC01000006.hg.1 12.5701
TC01000007.hg.1 12.62319
TC01000008.hg.1 8.406836
TC01000009.hg.1 3.945679
TC01000010.hg.1 5.145102
TC01000011.hg.1 7.389034
TC01000012.hg.1 5.727864
TC01000013.hg.1 10.53204
TC01000014.hg.1 4.301835
TC01000015.hg.1 7.342574
TC01000016.hg.1 7.162934
TC01000017.hg.1 7.140453
TC01000018.hg.1 8.94239
TC01000019.hg.1 7.310658
TC01000020.hg.1 8.447795

Total number of rows: 70523

Table truncated, full table size 1724 Kbytes.




Supplementary file Size Download File type/resource
GSM1536290_051314_TW_10_EPO41_HTA-2_0_.CEL.gz 27.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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