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Sample GSM1540965 Query DataSets for GSM1540965
Status Public on Mar 31, 2015
Title Act-DETC
Sample type SAGE
Anchor NlaIII
Tag Count 60970
Tag Length 10
Source name DETC, activated with anti-CD3
Organism Mus musculus
Characteristics cell type: DETC, activated with anti-CD3
strain: C57BL/6
Treatment protocol DETC were isolated from murine epidermis, stimulated in vitro on anti-CD3 coated plates (1 μg/ml) for 24 hours, cultured in IL-2 for four weeks, then restimulated with 1 μg/ml plate-bound anti-CD3 for 24 hours.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with an RNeasy RNA isolation kit from Qiagen and processed as in the supplied protocol (see also Shires et al (2001), Immunity, 15, 419-34
Complete SAGE protocol provided in Series supplementary file Hayday_SAGE_Protocol_Nov 2001.pdf
Data processing supplementary files include data normalized to 100,000 tags
Analysis was performed using SAGE300 and eSAGE software
Submission date Nov 07, 2014
Last update date Mar 31, 2015
Contact name Lucie Abeler-Dörner
Organization name King's College London
Department Immunobiology
Lab Hayday
Street address Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
Platform ID GPL11
Series (1)
GSE63090 Gene expression of conventional and unconventional T cell subsets

Supplementary file Size Download File type/resource
GSM1540965_Hayday_SAGE_library_5.txt.gz 22.1 Kb (ftp)(http) TXT
Processed data provided as supplementary file

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