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| Status |
Public on Mar 31, 2015 |
| Title |
Act-DETC |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
60970 |
| Tag Length |
10 |
| |
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| Source name |
DETC, activated with anti-CD3
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| Organism |
Mus musculus |
| Characteristics |
cell type: DETC, activated with anti-CD3
strain: C57BL/6
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| Treatment protocol |
DETC were isolated from murine epidermis, stimulated in vitro on anti-CD3 coated plates (1 μg/ml) for 24 hours, cultured in IL-2 for four weeks, then restimulated with 1 μg/ml plate-bound anti-CD3 for 24 hours.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with an RNeasy RNA isolation kit from Qiagen and processed as in the supplied protocol (see also Shires et al (2001), Immunity, 15, 419-34
Complete SAGE protocol provided in Series supplementary file Hayday_SAGE_Protocol_Nov 2001.pdf
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| Data processing |
supplementary files include data normalized to 100,000 tags
Analysis was performed using SAGE300 and eSAGE software
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| Submission date |
Nov 07, 2014 |
| Last update date |
Mar 31, 2015 |
| Contact name |
Lucie Abeler-Dörner |
| Organization name |
King's College London
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| Department |
Immunobiology
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| Lab |
Hayday
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| Street address |
Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platform ID |
GPL11 |
| Series (1) |
| GSE63090 |
Gene expression of conventional and unconventional T cell subsets |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM1540965_Hayday_SAGE_library_5.txt.gz |
22.1 Kb |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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