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Sample GSM154431 Query DataSets for GSM154431
Status Public on Mar 08, 2007
Title Yeast magnesium starvation, 90 minutes 2a
Sample type RNA
 
Channel 1
Source name yeast cells 90 min Mg starv
Organism Saccharomyces cerevisiae
Characteristics strain BY4741
Treatment protocol 90 min Mg starvation
Growth protocol yeast cultures were grown in synthetic medium containing 1 mM MgCl2 to OD600 = 0.5. The cultures were centrifuged, washed twice with pre-warmed synthetic medium containing no MgCl2 (-Mg2+), and resuspended in the same medium. cells were harvested after 90 minutes.
Extracted molecule total RNA
Extraction protocol Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and and K. Struhl. 1997. Current Protocols in Molecular Biology, pp. 13.12.1-13.12.5, J. Wiley and Sons, New York.
Label cy5
Label protocol as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
 
Channel 2
Source name yeast cells 90 min control
Organism Saccharomyces cerevisiae
Characteristics strain BY4741
Treatment protocol 90 min control
Growth protocol yeast cultures were grown in synthetic medium containing 1 mM MgCl2 to OD600 = 0.5. The cultures were centrifuged, washed twice with pre-warmed synthetic medium containing 1mM MgCl2 (+Mg2+), and resuspended in the same medium. cells were harvested after 90 minutes.
Extracted molecule total RNA
Extraction protocol Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and and K. Struhl. 1997. Current Protocols in Molecular Biology, pp. 13.12.1-13.12.5, J. Wiley and Sons, New York.
Label cy3
Label protocol as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
 
 
Hybridization protocol as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
Scan protocol Microarrays were read using an axon GenePix 4000B laser scanner (Axon Instruments) and analyzed with the GenePix Pro 3.0 software.
Description none
Data processing Ratio of Medians (635/532)
 
Submission date Jan 09, 2007
Last update date Mar 08, 2007
Contact name Jochen A Stadler
E-mail(s) jochen.stadler@univie.ac.at
Phone 0043-1-4277-54618
Organization name MFPL, University of Vienna
Department Department of Genetics
Street address Dr. Bohrgasse 9
City Vienna
ZIP/Postal code A1030
Country Austria
 
Platform ID GPL4734
Series (1)
GSE6687 Yeast magnesium starvation, 90 minutes

Data table header descriptions
ID_REF
VALUE log2 of PRE_VALUE
PRE_VALUE Ratio of Medians (635/532)

Data table
ID_REF VALUE PRE_VALUE
1 0.6924 1.616
2 0.4394 1.356
3 0.5724 1.487
4 0.4255 1.343
5 0.3380 1.264
6 0.5936 1.509
7 0.8584 1.813
8 -0.5995 0.66
9 -3.7959 0.072
10 -0.4189 0.748
11 1.2129 2.318
12 0.3516 1.276
13 null null
14 0.5529 1.467
15 0.8789 1.839
16 0.4657 1.381
17 null null
18 0.3528 1.277
19 0.4071 1.326
20 0.6406 1.559

Total number of rows: 6218

Table truncated, full table size 105 Kbytes.




Supplementary file Size Download File type/resource
GSM154431.gpr.gz 1.1 Mb (ftp)(http) GPR

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