yeast cultures were grown in synthetic medium containing 1 mM MgCl2 to OD600 = 0.5. The cultures were centrifuged, washed twice with pre-warmed synthetic medium containing no MgCl2 (-Mg2+), and resuspended in the same medium. cells were harvested after 90 minutes.
Extracted molecule
total RNA
Extraction protocol
Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and and K. Struhl. 1997. Current Protocols in Molecular Biology, pp. 13.12.1-13.12.5, J. Wiley and Sons, New York.
Label
cy5
Label protocol
as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
yeast cultures were grown in synthetic medium containing 1 mM MgCl2 to OD600 = 0.5. The cultures were centrifuged, washed twice with pre-warmed synthetic medium containing 1mM MgCl2 (+Mg2+), and resuspended in the same medium. cells were harvested after 90 minutes.
Extracted molecule
total RNA
Extraction protocol
Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and and K. Struhl. 1997. Current Protocols in Molecular Biology, pp. 13.12.1-13.12.5, J. Wiley and Sons, New York.
Label
cy3
Label protocol
as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
Hybridization protocol
as described at the UHN Microarray Center (Toronto, CA) website (ver. 2004.11.04)
Scan protocol
Microarrays were read using an axon GenePix 4000B laser scanner (Axon Instruments) and analyzed with the GenePix Pro 3.0 software.