|
Status |
Public on Jun 30, 2007 |
Title |
Bovine BL3 cells under normal culture conditions |
Sample type |
SAGE |
Anchor |
NlaIII
|
Tag Count |
72875 |
Tag Length |
10 |
|
|
Source name |
Bovine BL3 cells
|
Organism |
Bos taurus |
Characteristics |
Bovine BL3 cells - B cell lymphosarcoma cell line, nonadherent.
|
Treatment protocol |
Normal cells grown under normal culture conditions.
|
Growth protocol |
BL3 cells were grown in Leibovitz MEM with Earles salts medium supplemented with 1.4 mM glutamate.
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were collected by centrifugation and washed once with PBS. The cell pellet was resuspended and the cells lysed by addition of Trizol Reagent. The total RNA was prepared according to manufacturer's specifications.
|
|
|
Description |
SAGE library construction was done using the microSAGE protocol.
|
Data processing |
Tag sequences were extracted using SAGE 2000 ver. 4.0 software.
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|
|
Submission date |
Jan 10, 2007 |
Last update date |
Nov 19, 2008 |
Contact name |
John D. Neill |
E-mail(s) |
john.neill@ars.usda.gov
|
Phone |
515-663-7730
|
Organization name |
National Animal Disease Center
|
Lab |
VPDLRU
|
Street address |
2300 Dayton Ave.
|
City |
Ames |
State/province |
IA |
ZIP/Postal code |
50010 |
Country |
USA |
|
|
Platform ID |
GPL223 |
Series (1) |
GSE6704 |
Gene expression changes in BVDV2-infected BL3 cells |
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