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Sample GSM1547614 Query DataSets for GSM1547614
Status Public on Dec 07, 2016
Title 7-day-old seedlings of HSFA6b-OE at control condition, biological rep1
Sample type RNA
Source name 7-day-old seedling of HSFA6b-OE at control condition
Organism Arabidopsis thaliana
Characteristics genotype: HSFA6b-overexpression
tissue: seedling
age: 7-day-old
treatment: none
Treatment protocol 7-day-old seedlings were treated with 150mM NaCl for 6hr or 37℃ heat shock for 1hr, with 22℃ treatment as a control, then collected for RNA extracted.
Growth protocol Seedlings were grown at 22℃ on 0.5X MS plates containing 1% sucrose for 7 days.
Extracted molecule total RNA
Extraction protocol Samples were collected for RNA extraction by use of the RNeasy RNA extraction kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on the GeneChip Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
Description 7-day-old seedling of HSFA6b-OE at control condition
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Submission date Nov 17, 2014
Last update date Dec 07, 2016
Contact name Ya-Chen Huang
Organization name National Taiwan University
Department Institute of Plant Biology and Department of Life Science
Lab Tsung-Luo Jinn
Street address No. 1, Sec. 4, Roosevelt Road
City Taipei
ZIP/Postal code 10617
Country Taiwan
Platform ID GPL198
Series (1)
GSE63372 Expression data of HSFA6b-overexpression and HSFA6b dominant-negative mutant lines of Arabidopsis after NaCl or Heat shock treatment

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 338.2 P 0.000044
AFFX-BioB-M_at 483.5 P 0.000044
AFFX-BioB-3_at 407.6 P 0.000044
AFFX-BioC-5_at 1329.8 P 0.000044
AFFX-BioC-3_at 1154.9 P 0.000052
AFFX-BioDn-5_at 2426 P 0.000044
AFFX-BioDn-3_at 4581.9 P 0.000044
AFFX-CreX-5_at 10815.4 P 0.000044
AFFX-CreX-3_at 16196.9 P 0.000044
AFFX-DapX-5_at 56.3 P 0.000225
AFFX-DapX-M_at 152.8 P 0.000662
AFFX-DapX-3_at 369.2 P 0.00011
AFFX-LysX-5_at 15.2 A 0.262827
AFFX-LysX-M_at 43.1 A 0.108979
AFFX-LysX-3_at 85 P 0.00007
AFFX-PheX-5_at 21.9 A 0.368438
AFFX-PheX-M_at 11.3 A 0.300606
AFFX-PheX-3_at 34.9 P 0.042962
AFFX-ThrX-5_at 25.7 A 0.102145
AFFX-ThrX-M_at 43.6 P 0.042962

Total number of rows: 22810

Table truncated, full table size 589 Kbytes.

Supplementary file Size Download File type/resource
GSM1547614_BH0460-2.CEL.gz 2.0 Mb (ftp)(http) CEL
GSM1547614_BH0460-2.txt.gz 564.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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