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Sample GSM1547618 Query DataSets for GSM1547618
Status Public on Dec 07, 2016
Title 7-day-old seedlings of HSFA6b-RD treated with 37℃ heat shock for 1h, biological rep1
Sample type RNA
Source name 7-day-old seedlings of HSFA6b-RD treated with 37℃ heat shock for 1hr
Organism Arabidopsis thaliana
Characteristics genotype: HSFA6b dominant-negative
tissue: seedling
age: 7-day-old
treatment: 37℃ heat shock for 1hr
Treatment protocol 7-day-old seedlings were treated with 150mM NaCl for 6hr or 37℃ heat shock for 1hr, with 22℃ treatment as a control, then collected for RNA extracted.
Growth protocol Seedlings were grown at 22℃ on 0.5X MS plates containing 1% sucrose for 7 days.
Extracted molecule total RNA
Extraction protocol Samples were collected for RNA extraction by use of the RNeasy RNA extraction kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on the GeneChip Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
Description 7-day-old seedlings of HSFA6b-RD treated with 37℃ heat shock for 1hr
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Submission date Nov 17, 2014
Last update date Dec 07, 2016
Contact name Ya-Chen Huang
Organization name National Taiwan University
Department Institute of Plant Biology and Department of Life Science
Lab Tsung-Luo Jinn
Street address No. 1, Sec. 4, Roosevelt Road
City Taipei
ZIP/Postal code 10617
Country Taiwan
Platform ID GPL198
Series (1)
GSE63372 Expression data of HSFA6b-overexpression and HSFA6b dominant-negative mutant lines of Arabidopsis after NaCl or Heat shock treatment

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 881.4 P 0.000044
AFFX-BioB-M_at 1408.9 P 0.000044
AFFX-BioB-3_at 972.2 P 0.000044
AFFX-BioC-5_at 3137.7 P 0.000044
AFFX-BioC-3_at 2619.8 P 0.000052
AFFX-BioDn-5_at 5314.8 P 0.000044
AFFX-BioDn-3_at 9088.9 P 0.000044
AFFX-CreX-5_at 18591.9 P 0.000044
AFFX-CreX-3_at 27647.6 P 0.000044
AFFX-DapX-5_at 105.6 P 0.000297
AFFX-DapX-M_at 345.7 P 0.000754
AFFX-DapX-3_at 1165.4 P 0.000052
AFFX-LysX-5_at 39 P 0.009337
AFFX-LysX-M_at 69.4 P 0.002556
AFFX-LysX-3_at 196.3 P 0.000081
AFFX-PheX-5_at 30.4 A 0.123572
AFFX-PheX-M_at 44.8 P 0.039661
AFFX-PheX-3_at 144.5 P 0.000754
AFFX-ThrX-5_at 35.6 P 0.020022
AFFX-ThrX-M_at 77.6 P 0.002023

Total number of rows: 22810

Table truncated, full table size 587 Kbytes.

Supplementary file Size Download File type/resource
GSM1547618_BH0460-6.CEL.gz 2.0 Mb (ftp)(http) CEL
GSM1547618_BH0460-6.txt.gz 567.5 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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