|
| Status |
Public on Oct 29, 2015 |
| Title |
JSUB-2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse primary splenic B cells
|
| Organism |
Mus musculus |
| Characteristics |
cell types: B cells
cell subtypes: B10-
|
| Growth protocol |
all cells were cultured in RPMI1640 medium plus anti-Cd40 antibody for 48h and LPS+PMA+Ionomycin for the last 5h
stain cells with Cd19, Cd1d, Cd5 antibody and Il10 detection kit for cell sorting by FACSAria
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol, rRNA was depleted with the Ribo-Zero rRNA removal kit (Epicentre)
|
| Label |
cy3
|
| Label protocol |
standard LC Sciences protocol
|
| |
|
| Hybridization protocol |
standard LC Sciences protocol
|
| Scan protocol |
Scan with Axon GenePix 4000B Microarray Scanner, Pixel size is10 µm, sample A gain is 340
|
| Data processing |
standard LC Sciences protocol
|
| |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
Oct 29, 2015 |
| Contact name |
Jianbo Sun |
| E-mail(s) |
js4025@columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Microbiology and Immunology
|
| Lab |
Uttiya Basu
|
| Street address |
701 W 168th St, HHSC918
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL19427 |
| Series (1) |
| GSE63374 |
miRNA profiling for mouse B10+ cells and B10- B cells |
|
