|
| Status |
Public on Sep 15, 2015 |
| Title |
Sensitive 5.3 line |
| Sample type |
RNA |
| |
|
| Source name |
Sensitive 5.3 line
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: haematopoietic progenitor
cell line: 5.3 line
condition: Sensitive
|
| Treatment protocol |
Cell lines were grown in 0.1% DMSO or 400, 600, 800nM of I-BET151
|
| Growth protocol |
Cell lines were grown in RPMI-1640 supplemented with murine IL-3 (10 ng/mL), 20% fetal calf serum, penicillin (100 units/mL), streptomycin (100 ug/mL), amphotericin B (250ng/mL) and gentamycin (50 ug/mL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Qiagnen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: DMSO 5.3
|
| Data processing |
The data were normalized using quantile normalization in R
|
| |
|
| Submission date |
Nov 24, 2014 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Mark Dawson |
| E-mail(s) |
mark.dawson@petermac.org
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE63575 |
Genome wide expression analysis of BET inhibitor resistance |
| GSE63683 |
iBET resistance |
|
