|
Status |
Public on Sep 15, 2015 |
Title |
800 Resistant 5.1 line |
Sample type |
RNA |
|
|
Source name |
Resistant 5.1 line
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: haematopoietic progenitor cell line: 5.1 line condition: Resistant
|
Treatment protocol |
Cell lines were grown in 0.1% DMSO or 400, 600, 800nM of I-BET151
|
Growth protocol |
Cell lines were grown in RPMI-1640 supplemented with murine IL-3 (10 ng/mL), 20% fetal calf serum, penicillin (100 units/mL), streptomycin (100 ug/mL), amphotericin B (250ng/mL) and gentamycin (50 ug/mL)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using the Qiagnen RNeasy kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Sample name: 800 5.1
|
Data processing |
The data were normalized using quantile normalization in R
|
|
|
Submission date |
Nov 24, 2014 |
Last update date |
Sep 15, 2015 |
Contact name |
Mark Dawson |
E-mail(s) |
mark.dawson@petermac.org
|
Organization name |
Peter MacCallum Cancer Centre
|
Street address |
305 Grattan Street
|
City |
Melbourne |
State/province |
VIC |
ZIP/Postal code |
3000 |
Country |
Australia |
|
|
Platform ID |
GPL6887 |
Series (2) |
GSE63575 |
Genome wide expression analysis of BET inhibitor resistance |
GSE63683 |
iBET resistance |
|