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Sample GSM1552858 Query DataSets for GSM1552858
Status Public on Sep 15, 2015
Title 800 Resistant 5.1 line
Sample type RNA
 
Source name Resistant 5.1 line
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: haematopoietic progenitor
cell line: 5.1 line
condition: Resistant
Treatment protocol Cell lines were grown in 0.1% DMSO or 400, 600, 800nM of I-BET151
Growth protocol Cell lines were grown in RPMI-1640 supplemented with murine IL-3 (10 ng/mL), 20% fetal calf serum, penicillin (100 units/mL), streptomycin (100 ug/mL), amphotericin B (250ng/mL) and gentamycin (50 ug/mL)
Extracted molecule total RNA
Extraction protocol RNA was prepared using the Qiagnen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 800 5.1
Data processing The data were normalized using quantile normalization in R
 
Submission date Nov 24, 2014
Last update date Sep 15, 2015
Contact name Mark Dawson
E-mail(s) mark.dawson@petermac.org
Organization name Peter MacCallum Cancer Centre
Street address 305 Grattan Street
City Melbourne
State/province VIC
ZIP/Postal code 3000
Country Australia
 
Platform ID GPL6887
Series (2)
GSE63575 Genome wide expression analysis of BET inhibitor resistance
GSE63683 iBET resistance

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2417611 6.773639846 0.9241453
ILMN_2762289 6.975433668 0.09081197
ILMN_2896528 10.26847113 0
ILMN_2721178 8.380172189 0
ILMN_2458837 6.865051562 0.3899573
ILMN_3033922 9.627106697 0
ILMN_3092673 11.08810144 0
ILMN_1230777 10.77978851 0
ILMN_1246069 10.52156671 0
ILMN_1232042 6.786814385 0.8643162
ILMN_1243193 10.77613731 0
ILMN_2524361 6.902130862 0.2457265
ILMN_1242440 6.857280939 0.4348291
ILMN_1233188 6.750490781 0.9732906
ILMN_2543688 6.871240931 0.357906
ILMN_1259789 7.325185996 0
ILMN_2816356 7.01783663 0.0534188
ILMN_1224596 7.310908439 0
ILMN_1233643 6.926832567 0.1837607
ILMN_2808939 9.360197641 0

Total number of rows: 45281

Table truncated, full table size 1449 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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