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Sample GSM1558884 Query DataSets for GSM1558884
Status Public on May 29, 2015
Title TcdB_100pM_8hr_rep1
Sample type RNA
 
Source name Colonic epithelia, TcdB, 100pM, 8hr
Organism Homo sapiens
Characteristics cell type: cloned transverse colon stem cells differentiated in air-liquid interface culture
treatment: TcdB
treatment dosage: 100pM
treatment duration: 8hr
Treatment protocol Colonic stem cells differentiated in air-liquid interface cultures were treated with C. difficile toxin B at different doses (100 pM, 250 pM, and 500 pM) and durations (8 hrs, 16 hrs and 24 hrs).
Growth protocol Colonic epithelia was generated in air-liquid interface cultures from cloned transverse colon stem cells.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen).
Label Biotin
Label protocol EncoreĀ® Biotin Module (Nugen).
 
Hybridization protocol GeneChipĀ® Hybridization, Wash, and Stain Kit (Affymetrix).
Scan protocol GeneChipĀ® Hybridization, Wash, and Stain Kit (Affymetrix).
Description 100pM8hr-1
Colonic stem cells-air liquid interface.
Stem cell differentiation.
TcdB treatment.
Data processing RMA. Expression Console, Partek Genomics Suite 6.6.
Probe group file: HuEx-1_0-st-v2.r2.pgf
Meta-probeset file: HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
 
Submission date Dec 04, 2014
Last update date May 30, 2015
Contact name Frank McKeon
E-mail(s) mckeon.xian@gmail.com
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
 
Platform ID GPL5175
Series (2)
GSE63880 Modeling Clostridium difficile infections
GSE66749 Cloning and Variation of Ground State Intestinal Stem Cells Super [expression & SNP studies]

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
3622176 6.49443
3890640 6.40501
3544525 7.93406
3592196 5.18636
3191695 5.35681
3598613 3.6581
3759356 5.42828
2905404 6.37036
2378937 5.21873
2754673 6.64504
3756046 5.7937
3233605 6.07357
2931569 5.56745
3960174 7.36538
3934111 4.78862
3703885 5.84787
3022814 6.83018
2779271 7.44444
3403015 5.25302
3824993 8.05912

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.




Supplementary file Size Download File type/resource
GSM1558884_100pM8hr-1.CEL.gz 20.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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