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Status |
Public on May 29, 2015 |
Title |
TcdB_100pM_8hr_rep1 |
Sample type |
RNA |
|
|
Source name |
Colonic epithelia, TcdB, 100pM, 8hr
|
Organism |
Homo sapiens |
Characteristics |
cell type: cloned transverse colon stem cells differentiated in air-liquid interface culture treatment: TcdB treatment dosage: 100pM treatment duration: 8hr
|
Treatment protocol |
Colonic stem cells differentiated in air-liquid interface cultures were treated with C. difficile toxin B at different doses (100 pM, 250 pM, and 500 pM) and durations (8 hrs, 16 hrs and 24 hrs).
|
Growth protocol |
Colonic epithelia was generated in air-liquid interface cultures from cloned transverse colon stem cells.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Qiagen).
|
Label |
Biotin
|
Label protocol |
EncoreĀ® Biotin Module (Nugen).
|
|
|
Hybridization protocol |
GeneChipĀ® Hybridization, Wash, and Stain Kit (Affymetrix).
|
Scan protocol |
GeneChipĀ® Hybridization, Wash, and Stain Kit (Affymetrix).
|
Description |
100pM8hr-1 Colonic stem cells-air liquid interface. Stem cell differentiation. TcdB treatment.
|
Data processing |
RMA. Expression Console, Partek Genomics Suite 6.6. Probe group file: HuEx-1_0-st-v2.r2.pgf Meta-probeset file: HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
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|
|
Submission date |
Dec 04, 2014 |
Last update date |
May 30, 2015 |
Contact name |
Frank McKeon |
E-mail(s) |
mckeon.xian@gmail.com
|
Phone |
8608372486
|
Organization name |
University of Houston
|
Department |
Biology and Biochemistry
|
Street address |
3455 Cullen Blvd
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77004 |
Country |
USA |
|
|
Platform ID |
GPL5175 |
Series (2) |
GSE63880 |
Modeling Clostridium difficile infections |
GSE66749 |
Cloning and Variation of Ground State Intestinal Stem Cells Super [expression & SNP studies] |
|