|
Status |
Public on Jan 31, 2019 |
Title |
4_4mg_Cl_12h |
Sample type |
RNA |
|
|
Source name |
mRNA from pooled whole zebrafish embryos, labeled with Cyanine-3 (green)
|
Organism |
Danio rerio |
Characteristics |
tissue: whole embryo treatment: PCE time point: 12h concentration: 4mg nano: CI treated with nanomaterial (Carbo Iron 10 mg/l) batch: four
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol followed by RNeasy MiniKit cleanup (Qiagen) using the manufacturers’ protocols
|
Label |
Cy3
|
Label protocol |
One-Color Quick Amp Labeling Kit, according to manufacturer's protocol (Agilent Technologies, Waldbronn, Germany)
|
|
|
Hybridization protocol |
One-Color Quick Amp Labeling Kit, according to manufacturer's protocol (Agilent Technologies, Waldbronn, Germany)
|
Scan protocol |
Agilent DNA Microarray Scanner and Agilent Feature Extraction Software V11.5.1.1, according to manufacturer's protocol.
|
Data processing |
Background subtraction, Quantile normalization between arrays Software for data normalization: R software for windows (V2.15) package limma (V3.16.5)
|
|
|
Submission date |
Dec 09, 2014 |
Last update date |
Jan 31, 2019 |
Contact name |
Andreas SchĂĽttler |
E-mail(s) |
schuettler@bafg.de
|
Organization name |
German Federal Institute of Hydrology
|
Department |
Department Biochemistry and Ecotoxicology
|
Street address |
Am Mainzer Tor 1
|
City |
Koblenz |
ZIP/Postal code |
56068 |
Country |
Germany |
|
|
Platform ID |
GPL19526 |
Series (1) |
GSE63994 |
Concentration dependent gene expression in zebrafish embryos after PCE exposure |
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