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Sample GSM1566625 Query DataSets for GSM1566625
Status Public on Nov 25, 2021
Title hippocampus control biological rep1
Sample type RNA
 
Source name hippocampus, Control diet
Organism Mus musculus
Characteristics strain: C57BL/6
gender: male
diet: control
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Dec 16, 2014
Last update date Nov 25, 2021
Contact name Jamie Barger
E-mail(s) jamie.l.barger@gmail.com
Organization name LifeGen Technologies, LLC
Street address 510 Charmany Drive, Suite 263
City Madison
State/province WI
ZIP/Postal code 53719
Country USA
 
Platform ID GPL81
Series (1)
GSE64226 Gene expression in mouse hippocampus in response to long-term calorie restriction

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 34.1628 A 0.843268
AFFX-MurIL10_at 92.0787 A 0.52976
AFFX-MurIL4_at 30.7361 A 0.39692
AFFX-MurFAS_at 98.162 A 0.205732
AFFX-BioB-5_at 925.107 P 0.00844047
AFFX-BioB-M_at 1149.06 P 4.42873e-05
AFFX-BioB-3_at 828.013 P 0.000169227
AFFX-BioC-5_at 2668.07 P 0.000169227
AFFX-BioC-3_at 1898.64 P 4.42873e-05
AFFX-BioDn-5_at 2384.2 P 5.16732e-05
AFFX-BioDn-3_at 11144.8 P 0.00010954
AFFX-CreX-5_at 22911.3 P 4.42873e-05
AFFX-CreX-3_at 33678 P 4.42873e-05
AFFX-BioB-5_st 37.3081 A 0.41138
AFFX-BioB-M_st 115.55 A 0.559354
AFFX-BioB-3_st 36.4704 A 0.904333
AFFX-BioC-5_st 35.5037 A 0.916408
AFFX-BioC-3_st 21.4027 A 0.860518
AFFX-BioDn-5_st 60.6245 A 0.275146
AFFX-BioDn-3_st 193.216 A 0.262827

Total number of rows: 12488

Table truncated, full table size 361 Kbytes.




Supplementary file Size Download File type/resource
GSM1566625_hippocampus_control_1.cel.gz 2.6 Mb (ftp)(http) CEL
GSM1566625_hippocampus_control_1.mas5.CHP.gz 123.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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