|
| Status |
Public on Nov 25, 2021 |
| Title |
hippocampus control biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, Control diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
diet: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 16, 2014 |
| Last update date |
Nov 25, 2021 |
| Contact name |
Jamie Barger |
| E-mail(s) |
jamie.l.barger@gmail.com
|
| Organization name |
LifeGen Technologies, LLC
|
| Street address |
510 Charmany Drive, Suite 263
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53719 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE64226 |
Gene expression in mouse hippocampus in response to long-term calorie restriction |
|
