|
| Status |
Public on Dec 17, 2014 |
| Title |
stem cell line BG03B |
| Sample type |
genomic |
| |
|
| Source name |
stem cell line BG03B
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem cell
cell line: BG03
|
| Treatment protocol |
None
|
| Growth protocol |
Cells were grown on MEF feeders in standard conditions and separated using collagenase.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from embryonic stem cells using Qiagen Dneasy Tissue kit. DNA quality and quantity was assessed using a Tecan plate reader, PicoGreen and agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
Hybridized to Genome-Wide Human SNP Array 6.0
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software (GCOS version 4.0). Copy number values for individual SNPs were determined by Affymetrix Genotyping Console Software v.4.0.
chromosome, chromosome region start, chromosome region end, region=copy number region, signal=signal intensity, call=copy number state, confidence
|
| |
|
| Submission date |
Dec 16, 2014 |
| Last update date |
Dec 17, 2014 |
| Contact name |
William Freed |
| Organization name |
NIDA NIH
|
| Street address |
333 CASSELL DRIVE
|
| City |
BALTIMORE |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE64241 |
Affymetrix SNP copy number data for embryonic stem cell samples |
|
