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Sample GSM1586555 Query DataSets for GSM1586555
Status Public on Dec 23, 2017
Title Tumor, Splenic Diffuse Red Pulp Lymphoma, case 8
Sample type genomic
 
Channel 1
Source name Tumor, Splenic Diffuse Red Pulp Lymphoma, case 8
Organism Homo sapiens
Characteristics cell type: splenic diffuse red pulp lymphoma
gender: male
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted form formalin fixed paraffin embedded (FFPE) tissues by phenol chloroform method. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, and agarose gel electrophoresis.
Label Cy5
Label protocol Following manufacturer instructions (Agilent)
 
Channel 2
Source name pool normal DNA
Organism Homo sapiens
Characteristics gender: M
age: 20
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted form formalin fixed paraffin embedded (FFPE) tissues by phenol chloroform method. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, and agarose gel electrophoresis.
Label Cy3
Label protocol Following manufacturer instructions (Agilent)
 
 
Hybridization protocol The protocol Oligonucleotide Array-Based CGH for Genomic DNA Analysis (ULS labeling for Blood, Cells, Tissues or FFPE) was used.
Scan protocol Scanned on an Agilent G2565AA scanner.
Description Tumor vs normal
Data processing Raw data were generated from scanned images using Agilent Feature Extraction Software (10.10.1.1). Log2ratios of background corrected values for tumour over normal DNA were calculated. Normalization was carried out on Agilent's CGH Analytics, integrated on the Agilent Genomic Workbench Standard Edition 6.5.0.58, using data from the internal set of control probes included in the microarray. Detection of Copy Number Alterations (CNAs) was performed using the ADM-2 algorithm, also implemented within the Agilent's genomics suite Genomic Workbench v5.0, with a threshold of 6.5 and a minimum of 5 consecutive probes and Nexus 6.0 Discovery Edition (Biodiscovery, El segundo, CA).
 
Submission date Jan 16, 2015
Last update date Dec 23, 2017
Contact name Alba Navarro
E-mail(s) ANAVARRL@clinic.ub.es
Organization name IDIBAPS - Hospital ClĂ­nic
Department Pathology
Lab Molecular Pathology
Street address Villaroel, 170
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL8736
Series (2)
GSE64822 Clinical and molecular characterization of splenic diffuse red pulp lymphomas
GSE65060 Clinical and molecular characterization of splenic diffuse red pulp lymphomas [Agilent]

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
1 0.06738053337
2 0.2388035698
3 0
4 0.1253231851
5 0.05509838382
6 -0.1329853207
7 -0.07719399402
8 0.1102040734
9 0.02602153087
10 -0.08136623075
11 -0.106947635
12 0.009682566287
13 0.07965969774
14 0.007852798247
15 -0.004834928137
16 -0.09085264174
17 0.03256083279
18 -0.04515547908
19 0.158635336
20 0.1031753848

Total number of rows: 974016

Table truncated, full table size 19994 Kbytes.




Supplementary file Size Download File type/resource
GSM1586555_Case_8.txt.gz 268.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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